Human cord blood-derived cells generate insulin-producing cells in vivo

Shuro Yoshida, Fumihiko Ishikawa, Noriaki Kawano, Kazuya Shimoda, Seiho Nagafuchi, Shinji Shimoda, Masaki Yasukawa, Takaaki Kanemaru, Hiromi Ishibashi, Leonard D. Shultz, Mine Harada

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65% ± 0.64% in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.

Original languageEnglish
Pages (from-to)1409-1416
Number of pages8
JournalSTEM CELLS
Volume23
Issue number9
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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