Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation

Takeshi Sekiguchi, Hiroshi Iida, Junko Fukumura, Takeharu Nishimoto

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We investigated the function of DDX3Y, the Y chromosome AZFa region encoding a putative DEAD-box RNA helicase protein, the loss of which results in oligozoospermia or azoospermia in humans. The human DDX3Y amino acid sequence is similar to that of the X chromosome gene DDX3X (91.7% homology). Here we report that human Y- and X-encoded DEAD box RNA helicase proteins DDX3Y and DDX3X are interchangeable and have an essential function: both proteins rescued a temperature-sensitive mutant hamster cell line (tsET24) that was otherwise incapable of growth at a nonpermissive temperature. Mouse homologues Ddx3y and D1Pas1-PL10 also rescued the mutant cell line at a nonpermissive temperature. In situ hybridization revealed that Ddx3x mRNA was expressed in almost every cell in mouse testis, suggesting that Ddx3x is involved in spermatogenesis. A comparative study of DDX3X and DDX3Y was performed to determine the significance of DDX3Y for cell growth and spermatogenesis. Both DDX3X and DDX3Y promoter DNAs produced a similar degree of transcription in vivo, whereas deletion studies of the promoter DNAs indicated that these genes are differentially regulated. DDX3Y, similar to DDX3X, shuttles between the nucleus and cytoplasm in a crm1-dependent manner.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalExperimental Cell Research
Volume300
Issue number1
DOIs
Publication statusPublished - Oct 15 2004

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RNA Helicases
DEAD-box RNA Helicases
Cricetinae
Protein Isoforms
Spermatogenesis
Cell Line
Mutation
Temperature
Oligospermia
X-Linked Genes
Azoospermia
Proteins
Y Chromosome
DNA
Growth
In Situ Hybridization
Testis
Amino Acid Sequence
Cytoplasm
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation. / Sekiguchi, Takeshi; Iida, Hiroshi; Fukumura, Junko; Nishimoto, Takeharu.

In: Experimental Cell Research, Vol. 300, No. 1, 15.10.2004, p. 213-222.

Research output: Contribution to journalArticle

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