Human mitochondrial DNA is packaged with TFAM

Tanfis Istiaq Alam, Tomotake Kanki, Tsuyoshi Muta, Koutarou Ukaji, Yoshito Abe, Hiroshi Nakayama, Koji Takio, Naotaka Hamasaki, Dongchon Kang

Research output: Contribution to journalReview article

219 Citations (Scopus)

Abstract

Mitochondrial transcription factor A (TFAM), a member of the high mobility group proteins, is essential for maintenance of mitochondrial DNA (mtDNA). Most TFAM and mtDNA (both of which are normally soluble) was recovered from the particulate fraction of human placental mitochondria when extracted with the non-ionic detergent Nonidet P-40. mtDNA and TFAM were co-immunoprecipitated by anti-TFAM antibodies. TFAM was released into the supernatant by DNase I digestion of mtDNA in the particulate fraction. Thus, TFAM and mtDNA are tightly associated with each other, and it is likely that few TFAM or mtDNA molecules exist in an unbound form in mitochondria. Based on the fact that TFAM is abundant enough to wrap mtDNA entirely, these results suggest that human mtDNA is packaged with TFAM.

Original languageEnglish
Pages (from-to)1640-1645
Number of pages6
JournalNucleic acids research
Volume31
Issue number6
DOIs
Publication statusPublished - Mar 15 2003

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Alam, T. I., Kanki, T., Muta, T., Ukaji, K., Abe, Y., Nakayama, H., ... Kang, D. (2003). Human mitochondrial DNA is packaged with TFAM. Nucleic acids research, 31(6), 1640-1645. https://doi.org/10.1093/nar/gkg251