Human NAT10 is an ATP-dependent rna acetyltransferase responsible for N4-acetylcytidine formation in 18 S ribosomal RNA (rRNA)

Tsutomu Suzuki; Satoshi Ito; Sayuri Horikawa; Tateki Suzuki; Hiroki Kawauchi; Yoshikazu Tanaka; Takeo Suzuki

doi:10.1074/jbc.C114.602698

Human NAT10 is an ATP-dependent rna acetyltransferase responsible for N⁴-acetylcytidine formation in 18 S ribosomal RNA (rRNA)

Tsutomu Suzuki, Satoshi Ito, Sayuri Horikawa, Tateki Suzuki, Hiroki Kawauchi, Yoshikazu Tanaka, Takeo Suzuki

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N4-acetylcytidine (ac4C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAimediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac4C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

Original language	English
Pages (from-to)	35724-35730
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	289
Issue number	52
DOIs	https://doi.org/10.1074/jbc.C114.602698
Publication status	Published - Dec 26 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.C114.602698

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title = "Human NAT10 is an ATP-dependent rna acetyltransferase responsible for N4-acetylcytidine formation in 18 S ribosomal RNA (rRNA)",

abstract = "Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N4-acetylcytidine (ac4C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAimediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac4C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.",

author = "Tsutomu Suzuki and Satoshi Ito and Sayuri Horikawa and Tateki Suzuki and Hiroki Kawauchi and Yoshikazu Tanaka and Takeo Suzuki",

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T1 - Human NAT10 is an ATP-dependent rna acetyltransferase responsible for N4-acetylcytidine formation in 18 S ribosomal RNA (rRNA)

AU - Suzuki, Tsutomu

AU - Ito, Satoshi

AU - Horikawa, Sayuri

AU - Suzuki, Tateki

AU - Kawauchi, Hiroki

AU - Tanaka, Yoshikazu

AU - Suzuki, Takeo

PY - 2014/12/26

Y1 - 2014/12/26

N2 - Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N4-acetylcytidine (ac4C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAimediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac4C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

AB - Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N4-acetylcytidine (ac4C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAimediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac4C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

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Human NAT10 is an ATP-dependent rna acetyltransferase responsible for N⁴-acetylcytidine formation in 18 S ribosomal RNA (rRNA)

Abstract

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