Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress

Hiroshi Hayakawa, Aya Fujikane, Riyoko Ito, Masaki Matsumoto, Keiichi Nakayama, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Exposure of cells to oxygen radicals damage various biologically important molecules. Among the oxidized bases produced in nucleic acids, 8-oxo-7,8-dihydroguanine (8-oxoguanine) is particularly important since it causes base mispairing. To ensure accurate gene expression, organisms must have a mechanism to discriminate 8-oxoguanine-containing RNA from normal transcripts. We searched for proteins that specifically bind to 8-oxoguanine-containing RNA from human HeLa cell extracts, and the candidate proteins were identified using mass spectrometry. Among the identified candidates, splicing isoform 1 of heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) and splicing isoform C1 of heterogeneous nuclear ribonucleoprotein C1/C2 (HNRNPC) exhibited strong abilities to bind to oxidized RNA. The amount of HNRNPD protein rapidly decreased when cells were exposed to hydrogen peroxide, an agent that enhances oxidative stress. Moreover, the suppression of HNRNPD expression by siRNA caused cells to exhibit an increased sensitivity to hydrogen peroxide. The application of siRNA against HNRNPC also caused an increase in sensitivity to hydrogen peroxide. Since no additive effect was observed with a combined addition of siRNAs for HNRNPD and HNRNPC, we concluded that the two proteins may function in the same mechanism for the accurate gene expression.

Original languageEnglish
Pages (from-to)220-224
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume403
Issue number2
DOIs
Publication statusPublished - Dec 10 2010

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Oxidative stress
Heterogeneous-Nuclear Ribonucleoproteins
Oxidative Stress
RNA
Hydrogen Peroxide
Gene expression
Small Interfering RNA
Protein Isoforms
Proteins
Gene Expression
Cell Extracts
HeLa Cells
Nucleic Acids
Mass spectrometry
Reactive Oxygen Species
Mass Spectrometry
Molecules
8-hydroxyguanine
hnRNP D0

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress. / Hayakawa, Hiroshi; Fujikane, Aya; Ito, Riyoko; Matsumoto, Masaki; Nakayama, Keiichi; Sekiguchi, Mutsuo.

In: Biochemical and Biophysical Research Communications, Vol. 403, No. 2, 10.12.2010, p. 220-224.

Research output: Contribution to journalArticle

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