TY - JOUR
T1 - Human recombinant interleukin 1-mediated suppression of glycosaminoglycan synthesis in cultured rat costal chondrocytes
AU - Ikebe, Tetsuro
AU - Hirata, Masato
AU - Koga, Toshitaka
PY - 1986/10/15
Y1 - 1986/10/15
N2 - Effects of human recombinant interleukin 1 (IL-1) on the synthesis if glycosaminoglycan were examined with cultured rat costal chondrocytes. Incorporation of [35S]sulfate into glycosaminoglycan was strikingly diminished by the addition of IL-1 in a dose- and time- dependent manner. When the cells were cultured with 340 μg/ml of IL-1 for 72 hr, the synthesis of glycosaminoglycan was inhibited to 10% of the control. On the other hand, IL-1 had no effect on the morphology and proliferation of the chondrocytes. The suppression of glycosaminoglycan synthesis remained unchanged after the addition of indomethacin, indicating that the effect of IL-1 is independent of the enhanced synthesis of prostaglandins.
AB - Effects of human recombinant interleukin 1 (IL-1) on the synthesis if glycosaminoglycan were examined with cultured rat costal chondrocytes. Incorporation of [35S]sulfate into glycosaminoglycan was strikingly diminished by the addition of IL-1 in a dose- and time- dependent manner. When the cells were cultured with 340 μg/ml of IL-1 for 72 hr, the synthesis of glycosaminoglycan was inhibited to 10% of the control. On the other hand, IL-1 had no effect on the morphology and proliferation of the chondrocytes. The suppression of glycosaminoglycan synthesis remained unchanged after the addition of indomethacin, indicating that the effect of IL-1 is independent of the enhanced synthesis of prostaglandins.
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U2 - 10.1016/0006-291X(86)91102-2
DO - 10.1016/0006-291X(86)91102-2
M3 - Article
C2 - 3490846
AN - SCOPUS:0022967314
SN - 0006-291X
VL - 140
SP - 386
EP - 391
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -