Human recombinant interleukin 1-mediated suppression of glycosaminoglycan synthesis in cultured rat costal chondrocytes

Tetsuro Ikebe, Masato Hirata, Toshitaka Koga

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17 Citations (Scopus)

Abstract

Effects of human recombinant interleukin 1 (IL-1) on the synthesis if glycosaminoglycan were examined with cultured rat costal chondrocytes. Incorporation of [35S]sulfate into glycosaminoglycan was strikingly diminished by the addition of IL-1 in a dose- and time- dependent manner. When the cells were cultured with 340 μg/ml of IL-1 for 72 hr, the synthesis of glycosaminoglycan was inhibited to 10% of the control. On the other hand, IL-1 had no effect on the morphology and proliferation of the chondrocytes. The suppression of glycosaminoglycan synthesis remained unchanged after the addition of indomethacin, indicating that the effect of IL-1 is independent of the enhanced synthesis of prostaglandins.

Original languageEnglish
Pages (from-to)386-391
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume140
Issue number1
DOIs
Publication statusPublished - Oct 15 1986

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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