HVJ (Sendai virus)-cationic liposomes: A novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium

Y. Yonemitsu, Y. Kaneda, A. Muraishi, T. Yoshizumi, K. Sugimachi, K. Sueishi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We designed a novel technique for targeted gene transfer into the airway epithelium. This was constructed using multilamellar cationic liposomes, containing N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, phosphatidylcholine and cholesterol and fused with haemagglutinating virus of Japan (HVJ), namely HVJ cationic liposomes. Single aerosol delivery of this novel vector to the airway of rats led to a highly efficient and widespread transduction of fluorescein isothiocyanate-labeled oligonucleotides or lacZ gene into the bronchial epithelium and X alveolar macrophages, but not into the alveolar epithelium. The efficiency of gene transfer to the airway epithelium with a single administration of the lacZ gene was about 47.6% in the trachea, 39.0% in the bronchi and proximal bronchioli, and 2.9% in the terminal bronchioli, respectively (mean value, n = 6). Expression level of the luciferase gene delivered with this novel system was much higher than that without HVJ, in both the trachea and lung tissue. Two pretreatment HVJ-cationic liposome vehicles every other week resulted in minimal inflammatory infiltration in the subepithelial layer with no significant reduction in efficiency of the following gene transfer. We propose that this novel HVJ cationic liposome-mediated gene transfer system may be suitable for clinical gene therapy to treat subjects with lethal lung diseases such as cystic fibrosis.

Original languageEnglish
Pages (from-to)631-638
Number of pages8
JournalGene Therapy
Volume4
Issue number7
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

Sendai virus
Gene Transfer Techniques
Liposomes
Epithelium
Lac Operon
Trachea
Genes
Alveolar Macrophages
Bronchi
Aerosols
Luciferases
Fluorescein
Phosphatidylcholines
Cystic Fibrosis
Oligonucleotides
Genetic Therapy
Lung Diseases
Chlorides
Glutamic Acid
Cholesterol

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

HVJ (Sendai virus)-cationic liposomes : A novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium. / Yonemitsu, Y.; Kaneda, Y.; Muraishi, A.; Yoshizumi, T.; Sugimachi, K.; Sueishi, K.

In: Gene Therapy, Vol. 4, No. 7, 01.01.1997, p. 631-638.

Research output: Contribution to journalArticle

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