Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation

Jingjia Ye, Nana Shirakigawa, Hiroyuki Ijima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hepatocyte transplantation is a potential therapy for treating various liver diseases. However, oxygen shortage leading to loss of hepatocyte function becomes a limitation following hepatocyte transplantation. To overcome this problem, we developed a hybrid organoid, consisting of growth factor (GF)-immobilizable gel particles combined with hepatocytes. Benefits of the hybrid organoid were evaluated in three groups: (i) hybrid organoid consisting of cells and GF-immobilizable gel particles (HG-C); (ii) hybrid organoid consisting of cells and gel particles (G-C); and (iii) cells suspended in collagen (C-C). We found liver-specific functions of HG-C were maintained longer than in the other conditions during in vitro culture. Furthermore, after transplantation, HG-C was effective in maintaining viability of transplanted hepatocytes and promoting angiogenesis around the hepatocytes. In summary, transplantation of HG-C is a potential method for future liver tissue engineering.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number2
DOIs
Publication statusPublished - Aug 1 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Medicine(all)

Fingerprint Dive into the research topics of 'Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation'. Together they form a unique fingerprint.

  • Cite this