Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation

Jingjia Ye, Nana Shirakigawa, Hiroyuki Ijima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hepatocyte transplantation is a potential therapy for treating various liver diseases. However, oxygen shortage leading to loss of hepatocyte function becomes a limitation following hepatocyte transplantation. To overcome this problem, we developed a hybrid organoid, consisting of growth factor (GF)-immobilizable gel particles combined with hepatocytes. Benefits of the hybrid organoid were evaluated in three groups: (i) hybrid organoid consisting of cells and GF-immobilizable gel particles (HG-C); (ii) hybrid organoid consisting of cells and gel particles (G-C); and (iii) cells suspended in collagen (C-C). We found liver-specific functions of HG-C were maintained longer than in the other conditions during in vitro culture. Furthermore, after transplantation, HG-C was effective in maintaining viability of transplanted hepatocytes and promoting angiogenesis around the hepatocytes. In summary, transplantation of HG-C is a potential method for future liver tissue engineering.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number2
DOIs
Publication statusPublished - Aug 1 2015

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Organoids
Particles (particulate matter)
Extracellular Matrix
Hepatocytes
Gels
Transplantation
Liver
Intercellular Signaling Peptides and Proteins
Bioelectric potentials
Tissue Engineering
Tissue engineering
Collagen
Liver Diseases
Oxygen

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Medicine(all)

Cite this

Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation. / Ye, Jingjia; Shirakigawa, Nana; Ijima, Hiroyuki.

In: Journal of Bioscience and Bioengineering, Vol. 120, No. 2, 01.08.2015, p. 231-237.

Research output: Contribution to journalArticle

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