Hybridization-based DNA analysis by self-heating nanowire microfluidic devices

Hiromi Takahashi, Takao Yasui, Keiko Shinjo, Quanli Liu, Taisuke Shimada, Noritada Kaji, Hiromu Kashida, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Advancement in the molecular analysis has expanded our understandings in the medical research and diagnostic. Here we developed the self-heating nanowires microfluidic device for detecting target sequences from circulating free DNA (cfDNA). First, we confirmed that this device enable to denature double-stranded DNAs to single-stranded DNAs, then efficiently capture 89% of cfDNAs. Next, the full performance of this device was confirmed, which can denature, in-situ capture cfDNAs and hybridize probes with target DNAs within a device. Moreover, we also investigated the interaction between nanowires and cfDNA using FTIR analysis.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages720-721
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period10/27/1910/31/19

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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