Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

Atsuko Noda; Hiroshi Noda; Ayako Misaka; Hideki Sumimoto; Kiyoshi Tatsumi

doi:10.1016/S0006-291X(88)81216-6

Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

Atsuko Noda, Hiroshi Noda, Ayako Misaka, Hideki Sumimoto, Kiyoshi Tatsumi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O₂ and NADPH.

Original language	English
Pages (from-to)	256-260
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	153
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(88)81216-6
Publication status	Published - May 31 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(88)81216-6

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abstract = "Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O2 and NADPH.",

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T1 - Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

AU - Noda, Atsuko

AU - Noda, Hiroshi

AU - Misaka, Ayako

AU - Sumimoto, Hideki

AU - Tatsumi, Kiyoshi

PY - 1988/5/31

Y1 - 1988/5/31

N2 - Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O2 and NADPH.

AB - Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O2 and NADPH.

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Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

Abstract

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