Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

Atsuko Noda, Hiroshi Noda, Ayako Misaka, Hideki Sumimoto, Kiyoshi Tatsumi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O2 and NADPH.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume153
Issue number1
DOIs
Publication statusPublished - May 31 1988

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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