Hydrodynamic chromatography using monolithic silica capillary column

Koichi Ute, Saori Yoshida, Tatsuki Kitayama, Takeshi Bamba, Ei Ichiro Fukusaki, Akio Kobayashi, Hiroyoshi Minakuchi

Research output: Contribution to conferencePaperpeer-review

Abstract

Monolithic silica capillary column has been applied to the micro HPLC separation of polymers according to molecular weight. The monolithic silica in a fused silica capillary (200 μm i.d. × 150 cm) has a mesoporous structure (pore size = 20 nm) together with a μm-sized continuous through-pores. When THF was used as the mobile phase, 8 polystyrenes with M w from 7,450,000 to 2,900 were separated clearly (85,000 theoretical plates) and eluted in that order. The elution volume vs. log Mw plots showed excellent linearity. The pressure drop at a flow rate of 0.5 μL/min was 2.5 MPa. The maximum porosity exceeding 7,000,000 can not be explained by a size-exclusion effect of 20 nm mesopores.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
Country/TerritoryJapan
CityYokohama
Period5/25/055/27/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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