Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

Toyohito Naito, Noritada Kaji, Manabu Tokeshi, Takuya Kubo, Yoshinobu Baba, Koji Otsuka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel. DLD devices with low array angles collect floating S. cerevisiae through an array effectively. The DLD device with an array angle of 2.6°showed an efficiency of 91.7%. Two types of loop channels with a piezoelectrie pump make a stable two-phase laminar flow of a pre-filled fluid and supplied fluid. A loop channel with a connection between an inlet and outlet on the opposite side replaces a filled fluid in the channel with a supplied fluid, which is essential for supplying nutrient rich medium to cells in microfluidic channels as well as retaining cells in a microenvironment without external stresses.

Original languageEnglish
Pages (from-to)7264-7269
Number of pages6
JournalAnalytical Methods
Volume7
Issue number17
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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