Hydrodynamics of cryogelation

Keiichi Miyamoto, Yoshiyuki Shimonishi, Masayuki Tokita, Takashi Komai

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Abstract

Cryogel, prevalent in the plasma of rheumatoid arthritis patients, is a plasma fibronectin (pFN)-extra domain A containing FN [EDA(+)FN]-fibrinogen (Fbg) aggregate formed by the addition of heparin (Hep) at low temperature. Although EDA(+)FN is not usually present in normal plasma, its prevalence in rheumatic patients induces cryogelation. In this study, we determined the hydrodynamic radius (Rh) ratio (Rh/Rh30) of the cryogel component by dynamic light scattering in vitro. Rh/Rh30 was normalized to Rh at 30 °C (Rh30) at several temperatures. The Rh/Rh30 of Fbg was found to increase only by self-aggregation, whereas the Rh/Rh30 of FNs does not increase in response to temperature changes. The Rh/Rh30 of the Fbg/FN aggregate is increased by the addition of Hep, and the Rh/Rh30 (12.5) of the Hep-induced EDA(+)FN/Fbg aggregate is greater than that (2.5) of the pFN/Fbg aggregate. These results suggest that cryogelation requires Fbg self-aggregation and the interaction between EDA(+)FN and Hep.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume29
Issue number4-5
DOIs
Publication statusPublished - Dec 10 2001

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

Cite this

Miyamoto, K., Shimonishi, Y., Tokita, M., & Komai, T. (2001). Hydrodynamics of cryogelation. International Journal of Biological Macromolecules, 29(4-5), 219-223. https://doi.org/10.1016/S0141-8130(01)00168-4