Hydrogen and carbon isotopic fractionations of lipid biosynthesis among terrestrial (C3, C4 and CAM) and aquatic plants

Yoshito Chikaraishi, Hiroshi Naraoka, Simon R. Poulson

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.

Original languageEnglish
Pages (from-to)1369-1381
Number of pages13
JournalPhytochemistry
Volume65
Issue number10
DOIs
Publication statusPublished - May 1 2004
Externally publishedYes

Fingerprint

Phytol
isotope fractionation
Crassulacean acid metabolism
Biosynthesis
aquatic plants
Fractionation
Metabolism
Sterols
sterols
hydrogen
Hydrogen
Carbon
biosynthesis
Lipids
Acids
carbon
lipids
Carboxylic acids
Angiosperms
Phytosterols

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

Cite this

Hydrogen and carbon isotopic fractionations of lipid biosynthesis among terrestrial (C3, C4 and CAM) and aquatic plants. / Chikaraishi, Yoshito; Naraoka, Hiroshi; Poulson, Simon R.

In: Phytochemistry, Vol. 65, No. 10, 01.05.2004, p. 1369-1381.

Research output: Contribution to journalArticle

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