Abstract
Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.
| Original language | English |
|---|---|
| Pages (from-to) | 1369-1381 |
| Number of pages | 13 |
| Journal | Phytochemistry |
| Volume | 65 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - May 1 2004 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Plant Science
- Horticulture
Cite this
Hydrogen and carbon isotopic fractionations of lipid biosynthesis among terrestrial (C3, C4 and CAM) and aquatic plants. / Chikaraishi, Yoshito; Naraoka, Hiroshi; Poulson, Simon R.
In: Phytochemistry, Vol. 65, No. 10, 01.05.2004, p. 1369-1381.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Hydrogen and carbon isotopic fractionations of lipid biosynthesis among terrestrial (C3, C4 and CAM) and aquatic plants
AU - Chikaraishi, Yoshito
AU - Naraoka, Hiroshi
AU - Poulson, Simon R.
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.
AB - Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.
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U2 - 10.1016/j.phytochem.2004.03.036
DO - 10.1016/j.phytochem.2004.03.036
M3 - Article
C2 - 15231410
AN - SCOPUS:3042559899
VL - 65
SP - 1369
EP - 1381
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
IS - 10
ER -