TY - JOUR
T1 - Hydrogen and carbon isotopic fractionations of lipid biosynthesis among terrestrial (C3, C4 and CAM) and aquatic plants
AU - Chikaraishi, Yoshito
AU - Naraoka, Hiroshi
AU - Poulson, Simon R.
N1 - Funding Information:
We thank Mr. Naito for the assistance in the field. Thailand leaf samples were kindly donated by S. Ando of Japan International Research Center for Agricultural Sciences and Prof. T. Yoneyama of Uiversity of Tokyo. This work was supported by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (Y.C.) and the Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Culture and Technology (H.N.).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/5
Y1 - 2004/5
N2 - Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.
AB - Compound-specific hydrogen and carbon isotopic compositions in n-alkanoic acids, phytol and sterols were determined for various plant classes (terrestrial C3-angiosperm; C3-gymnosperm; C4; crassulacean acid metabolism (CAM); and aquatic C3 plants) in order to investigate isotopic fractionations among various plant classes. In all plants, lipid biomolecules are depleted in both D (up to 324‰) and 13C (up to 14.7‰) relative to ambient water and bulk tissue, respectively. In addition, the magnitude of D- and 13C-depletion of lipid biomolecules is distinctive depending on plant classes. For example, C3 angiosperm n-alkanoic acids are less depleted in D (95±23‰) and 13C (4.3±2.5‰) relative to ambient water and bulk tissue, respectively, while C4 plant n-alkanoic acids are more depleted in D (119±15‰) and 13C (10.2±2.0‰). On the other hand, C3 angiosperm phytol and sterols are much more depleted in D (306±12‰ for phytol, 211±15‰ for sterol) with less depletion in 13C (4.1±1.1‰ for phytol, 1.3±0.9‰ for sterol) relative to ambient water and bulk tissue, respectively, while C4 plant phytol and sterols are less depleted in D (254±7‰ for phytol, 186±13‰ for sterols) with much more depletion in 13C (9.0±1.2‰ for phytol, 5.0±1.1‰ for sterols). Among various plant classes, there is a positive correlation between the D- and 13C-depletion for n-alkanoic acids, while a negative correlation was found for phytol and sterols from the same plants.
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U2 - 10.1016/j.phytochem.2004.03.036
DO - 10.1016/j.phytochem.2004.03.036
M3 - Article
C2 - 15231410
AN - SCOPUS:3042559899
VL - 65
SP - 1369
EP - 1381
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
IS - 10
ER -