Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model

Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Shinsuke Mizutani, Azusa Kojima, Manabu Morita

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Abstract

Objective: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model. Design: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800-1000 μg/L hydrogen during the 4-wk experimental period. Results: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p < 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p < 0.05). Conclusions: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress.

Original languageEnglish
Pages (from-to)1615-1622
Number of pages8
JournalArchives of Oral Biology
Volume57
Issue number12
DOIs
Publication statusPublished - Dec 1 2012
Externally publishedYes

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Periodontitis
Thoracic Aorta
Hydrogen
Lipids
Water
LDL Cholesterol
Reactive Oxygen Species
Lysine
Aorta
Atherosclerosis
Oxidative Stress
Serum
Control Groups
Drinking
Ligation
Wistar Rats

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Ekuni, D., Tomofuji, T., Endo, Y., Kasuyama, K., Irie, K., Azuma, T., ... Morita, M. (2012). Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model. Archives of Oral Biology, 57(12), 1615-1622. https://doi.org/10.1016/j.archoralbio.2012.04.013

Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model. / Ekuni, Daisuke; Tomofuji, Takaaki; Endo, Yasumasa; Kasuyama, Kenta; Irie, Koichiro; Azuma, Tetsuji; Tamaki, Naofumi; Mizutani, Shinsuke; Kojima, Azusa; Morita, Manabu.

In: Archives of Oral Biology, Vol. 57, No. 12, 01.12.2012, p. 1615-1622.

Research output: Contribution to journalArticle

Ekuni, D, Tomofuji, T, Endo, Y, Kasuyama, K, Irie, K, Azuma, T, Tamaki, N, Mizutani, S, Kojima, A & Morita, M 2012, 'Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model', Archives of Oral Biology, vol. 57, no. 12, pp. 1615-1622. https://doi.org/10.1016/j.archoralbio.2012.04.013
Ekuni, Daisuke ; Tomofuji, Takaaki ; Endo, Yasumasa ; Kasuyama, Kenta ; Irie, Koichiro ; Azuma, Tetsuji ; Tamaki, Naofumi ; Mizutani, Shinsuke ; Kojima, Azusa ; Morita, Manabu. / Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model. In: Archives of Oral Biology. 2012 ; Vol. 57, No. 12. pp. 1615-1622.
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AU - Ekuni, Daisuke

AU - Tomofuji, Takaaki

AU - Endo, Yasumasa

AU - Kasuyama, Kenta

AU - Irie, Koichiro

AU - Azuma, Tetsuji

AU - Tamaki, Naofumi

AU - Mizutani, Shinsuke

AU - Kojima, Azusa

AU - Morita, Manabu

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N2 - Objective: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model. Design: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800-1000 μg/L hydrogen during the 4-wk experimental period. Results: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p < 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p < 0.05). Conclusions: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress.

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