Triolein, a major component of olive oil, was hydrolyzed with lipase in a membrane bioreactor using hollow fiber. The hydrolysis mechanism of triolein was investigated, taking account of the mass transfer of triolein and oleic acid through the membrane. It was made clear that oleic acid, one of the products, strongly inhibited the hydrolysis of triolein at high concentration. Glycerin, another product, did not inhibit the reaction. This hydrolysis reaction with lipase could be quantitatively explained by the Michaelis-Menten mechanism at the interface between the aqueous and organic phases, taking account of the adsorption of lipase and the permeation rate of triolein and oleic acid.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)