Hydrolysis Mechanism of Triolein with Lipase in Membrane Bioreactor using Hollow Fiber

Muneharu Goto, Masahiro Goto, Fumiyuki Nakashio, Kazuharu Yoshizuka, Katsutoshi Inoue, Yoshinari Baba

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Triolein, a major component of olive oil, was hydrolyzed with lipase in a membrane bioreactor using hollow fiber. The hydrolysis mechanism of triolein was investigated, taking account of the mass transfer of triolein and oleic acid through the membrane. It was made clear that oleic acid, one of the products, strongly inhibited the hydrolysis of triolein at high concentration. Glycerin, another product, did not inhibit the reaction. This hydrolysis reaction with lipase could be quantitatively explained by the Michaelis-Menten mechanism at the interface between the aqueous and organic phases, taking account of the adsorption of lipase and the permeation rate of triolein and oleic acid.

Original languageEnglish
Pages (from-to)477-483
Number of pages7
Journalkagaku kogaku ronbunshu
Volume17
Issue number3
DOIs
Publication statusPublished - Jan 1 1991

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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