Hydrolysis of Soluble Starch by Glucoamylase Immobilized on Ceramic Monolith

Fumihide Shiraishi, Koei Kawakami, Keiichi Kato, Koichiro Kusunoki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To evaluate the effectiveness of a ceramic monolith (mean pore diameter, 15 μm) as a support for the immobilized enzyme that catalyzes the hydrolysis of a high-molecular weight substrate, the hydrolysis of soluble starch (average molecular weight, 30 000) by use of glucoamylase from Rhizopus delemar was carried out at 50°C, pH 4.5. By observing the initial reaction rate in a batch reactor, wherein a fan-shaped piece of the immobilized enzyme monolith was fixed around a stirrer shaft, the pore diffusion resistance was found to be negligible when the concentration of starch exceeds 89 g/l. The simplified kinetic model, previously proposed, that assumed kinetic parameters as changing linearly with reduction of average molecular weight of substrate was also useful for simulating the immobilized enzyme system, regardless of batch or continuous mode, described in this paper.

Original languageEnglish
Pages (from-to)316-323
Number of pages8
Journalkagaku kogaku ronbunshu
Volume9
Issue number3
DOIs
Publication statusPublished - Jan 1 1983

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Glucan 1,4-alpha-Glucosidase
Immobilized Enzymes
Starch
Hydrolysis
Enzymes
Molecular weight
Batch reactors
Substrates
Kinetic parameters
Catalyst supports
Fans
Reaction rates
Kinetics

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Hydrolysis of Soluble Starch by Glucoamylase Immobilized on Ceramic Monolith. / Shiraishi, Fumihide; Kawakami, Koei; Kato, Keiichi; Kusunoki, Koichiro.

In: kagaku kogaku ronbunshu, Vol. 9, No. 3, 01.01.1983, p. 316-323.

Research output: Contribution to journalArticle

Shiraishi, Fumihide ; Kawakami, Koei ; Kato, Keiichi ; Kusunoki, Koichiro. / Hydrolysis of Soluble Starch by Glucoamylase Immobilized on Ceramic Monolith. In: kagaku kogaku ronbunshu. 1983 ; Vol. 9, No. 3. pp. 316-323.
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