Hydrothermal synthesis of porous hydroxyapatite plates from gypsum

Use as an animal cell culture substratum

Hiroyuki Ijima, Motonori Nakashima, Sachiko Furuta, Tsutomu Ono, Koei Kawakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

Original languageEnglish
Pages (from-to)531-535
Number of pages5
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume37
Issue number4
DOIs
Publication statusPublished - Apr 1 2004

Fingerprint

Animal cell culture
Calcium Sulfate
Hydrothermal synthesis
Gypsum
Durapatite
Bioreactors
Hydroxyapatite
Packed beds
Cell culture
Porosity
CHO Cells

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Hydrothermal synthesis of porous hydroxyapatite plates from gypsum : Use as an animal cell culture substratum. / Ijima, Hiroyuki; Nakashima, Motonori; Furuta, Sachiko; Ono, Tsutomu; Kawakami, Koei.

In: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, Vol. 37, No. 4, 01.04.2004, p. 531-535.

Research output: Contribution to journalArticle

Ijima, Hiroyuki ; Nakashima, Motonori ; Furuta, Sachiko ; Ono, Tsutomu ; Kawakami, Koei. / Hydrothermal synthesis of porous hydroxyapatite plates from gypsum : Use as an animal cell culture substratum. In: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN. 2004 ; Vol. 37, No. 4. pp. 531-535.
@article{c7afbfea722747e287b36b183726d221,
title = "Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum",
abstract = "A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28{\%} that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.",
author = "Hiroyuki Ijima and Motonori Nakashima and Sachiko Furuta and Tsutomu Ono and Koei Kawakami",
year = "2004",
month = "4",
day = "1",
doi = "10.1252/jcej.37.531",
language = "English",
volume = "37",
pages = "531--535",
journal = "Journal of Chemical Engineering of Japan",
issn = "0021-9592",
publisher = "Society of Chemical Engineers, Japan",
number = "4",

}

TY - JOUR

T1 - Hydrothermal synthesis of porous hydroxyapatite plates from gypsum

T2 - Use as an animal cell culture substratum

AU - Ijima, Hiroyuki

AU - Nakashima, Motonori

AU - Furuta, Sachiko

AU - Ono, Tsutomu

AU - Kawakami, Koei

PY - 2004/4/1

Y1 - 2004/4/1

N2 - A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

AB - A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

UR - http://www.scopus.com/inward/record.url?scp=3042646239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042646239&partnerID=8YFLogxK

U2 - 10.1252/jcej.37.531

DO - 10.1252/jcej.37.531

M3 - Article

VL - 37

SP - 531

EP - 535

JO - Journal of Chemical Engineering of Japan

JF - Journal of Chemical Engineering of Japan

SN - 0021-9592

IS - 4

ER -