Hydroxyl radical generation in skeletal muscle atrophied by immobilization

H Kondo, K Nishino, Y Itokawa

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Male Wistar rats (15 weeks old), the ankle joints of one hindlimb of which were immobilized in the extended position for 12 days, were injected with salicylate as a trapping agent for hydroxyl radicals before sacrifice. Atrophied and contralateral soleus, typical slow red muscles were collected and their levels of 2,3-dihydroxybenzoic acid (2,3-DHB), one of the main reaction products formed by the attack of hydroxyl radical on salicylate, were determined using high-performance liquid chromatography with an electrochemical detector. There was a significant increase in 2,3-DHB in the atrophied muscle. This result strongly suggests the enhanced generation in vivo of hydroxyl radicals in atrophied muscle.

Original languageEnglish
Pages (from-to)169-72
Number of pages4
JournalFEBS Letters
Volume349
Issue number2
Publication statusPublished - Aug 1 1994

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Immobilization
Hydroxyl Radical
Muscle
Skeletal Muscle
Salicylates
Muscles
Ankle Joint
High performance liquid chromatography
Hindlimb
Reaction products
Wistar Rats
Rats
High Pressure Liquid Chromatography
Detectors
2,3-dihydroxybenzoic acid

Cite this

Hydroxyl radical generation in skeletal muscle atrophied by immobilization. / Kondo, H; Nishino, K; Itokawa, Y.

In: FEBS Letters, Vol. 349, No. 2, 01.08.1994, p. 169-72.

Research output: Contribution to journalArticle

Kondo, H, Nishino, K & Itokawa, Y 1994, 'Hydroxyl radical generation in skeletal muscle atrophied by immobilization', FEBS Letters, vol. 349, no. 2, pp. 169-72.
Kondo, H ; Nishino, K ; Itokawa, Y. / Hydroxyl radical generation in skeletal muscle atrophied by immobilization. In: FEBS Letters. 1994 ; Vol. 349, No. 2. pp. 169-72.
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