Hyperbaric oxygenation prevents delayed neuronal death following transient ischaemia in the gerbil hippocampus

A. Kondo, S. Baba, T. Iwakit, H. Harai, H. Koga, T. Kimura, J. Takamatsu

Research output: Contribution to journalArticle

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Abstract

The mechanism of the neuroprotective effect of hyperbaric oxygenation remains unclear although its clinical benefits have been well recognized for human ischaemic neuronal disease. The preventive effect of hyperbaric oxygenation against delayed neuronal death was investigated in the gerbil following transient forebrain ischaemia. Delayed neuronal death in the gerbil was produced by clips on both the common carotid arteries (10 min). Morphological examination was carried out after several protocols of hyperbaric oxygenation, modified from the protocols for human ischaemic neuronal disease. Neurons in the hippocampal CA1 were well preserved in the gerbils treated with hyperbaric oxygenation, more so than in the gerbils with no hyperbaric oxygenation. Moreover, more neurons were preserved in the CA1 treated with hyperbaric oxygenation within 6h of the ischaemia, than when the hyperbaric oxygenation was started 24h after the ischaemia. The induction of heat shock proteins (HSP72 and HSP27) became weaker in the gerbils with hyperbaric oxygenation than in those without hyperbaric oxygenation, as seen immunohistochemically. We also observed an increase in dense bodies, that were shown to be lysosomes and myelinoid structures in the cytoplasm of the neurons ultrastructurally, in the hippocampus with hyperbaric oxygenation. However, no oxygen toxicity to the neurons was detected, up to at least two atmospheres absolute. This experimental system was useful to investigate the preventive mechanism of hyperbaric oxygenation against delayed neuronal death in the gerbil, and to determine the clinical indications and the most effective protocol for hyperbaric oxygenation for ischaemic neuronal damage in the human brain.

Original languageEnglish
Pages (from-to)350-360
Number of pages11
JournalNeuropathology and Applied Neurobiology
Volume22
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

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Hyperbaric Oxygenation
Gerbillinae
Hippocampus
Ischemia
Neurons
HSP72 Heat-Shock Proteins
HSP27 Heat-Shock Proteins
Common Carotid Artery
Neuroprotective Agents
Prosencephalon
Lysosomes
Atmosphere
Surgical Instruments
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

Cite this

Hyperbaric oxygenation prevents delayed neuronal death following transient ischaemia in the gerbil hippocampus. / Kondo, A.; Baba, S.; Iwakit, T.; Harai, H.; Koga, H.; Kimura, T.; Takamatsu, J.

In: Neuropathology and Applied Neurobiology, Vol. 22, No. 4, 01.01.1996, p. 350-360.

Research output: Contribution to journalArticle

Kondo, A. ; Baba, S. ; Iwakit, T. ; Harai, H. ; Koga, H. ; Kimura, T. ; Takamatsu, J. / Hyperbaric oxygenation prevents delayed neuronal death following transient ischaemia in the gerbil hippocampus. In: Neuropathology and Applied Neurobiology. 1996 ; Vol. 22, No. 4. pp. 350-360.
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