Hyperspectral coherent Raman imaging - Principle, theory, instrumentation, and applications to life sciences

Hideaki Kano; Hiroki Segawa; Masanari Okuno; Philippe Leproux; Vincent Couderc

doi:10.1002/jrs.4853

Hyperspectral coherent Raman imaging - Principle, theory, instrumentation, and applications to life sciences

Hideaki Kano, Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc

Research output: Contribution to journal › Review article › peer-review

24 Citations (Scopus)

Abstract

Coherent Raman scattering microscopy such as coherent anti-Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label-free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences.

Original language	English
Pages (from-to)	116-123
Number of pages	8
Journal	Journal of Raman Spectroscopy
Volume	47
Issue number	1
DOIs	https://doi.org/10.1002/jrs.4853
Publication status	Published - Jan 1 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

Materials Science(all)
Spectroscopy

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10.1002/jrs.4853

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AU - Kano, Hideaki

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AU - Leproux, Philippe

AU - Couderc, Vincent

PY - 2016/1/1

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AB - Coherent Raman scattering microscopy such as coherent anti-Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label-free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences.

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Hyperspectral coherent Raman imaging - Principle, theory, instrumentation, and applications to life sciences

Abstract

All Science Journal Classification (ASJC) codes

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