Hyperspectral coherent Raman imaging - Principle, theory, instrumentation, and applications to life sciences

Hideaki Kano, Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc

Research output: Contribution to journalReview articlepeer-review

21 Citations (Scopus)

Abstract

Coherent Raman scattering microscopy such as coherent anti-Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label-free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalJournal of Raman Spectroscopy
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Spectroscopy

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