Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks

Vishwajit Surchowdhury, Shozo Tomonaga, Shotaro Nishimura, Shoji Tabata, John F. Cockrem, Kazuyoshi Tsutsui, Mitsuhiro Furuse

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Abstract

The regulation of food intake in chickens (. Gallus gallus domesticus) represents a complex homeostatic mechanism involving multiple levels of control, and regulation during high ambient temperatures (HT) is poorly understood. In this study, we examined hypothalamic mRNA expression of gonadotropin-inhibitory hormone (GnIH) to understand the effect of HT on an orexigenic neuropeptide. We examined the effects of HT (35. °C ambient temperature for 1, 24 or 48. h) on 14-day old chicks. HT significantly increased rectal temperature and suppressed food intake, and also influenced plasma metabolites. The expression of GnIH precursor mRNA in the diencephalon was significantly increased in chicks at 24-and 48. h of HT when food intake was suppressed significantly, whilst no change was observed for GnIH precursor mRNA and food intake at 1. h of HT. . In situ hybridization and immunocytochemistry further revealed the cellular localization of chicken GnIH precursor mRNA and its peptide in the paraventricular nucleus (PVN) in the chick hypothalamus. We examined plasma metabolites in chicks exposed to HT for 1 or 48. h and found that triacylglycerol concentration was significantly higher in HT than control chicks at 1. h. Total protein, uric acid and calcium were significantly lower in HT chicks than control chicks at 48. h. These results indicate that not only a reduction in food intake and alteration in plasma metabolites but also the PVN-specific expression of GnIH, an orexigenic agent, may be induced by HT. The reduced food intake at the same time as GnIH expression was increased during HT suggests that HT-induced GnIH expression may oppose HT-induced feeding suppression, rather than promote it. We suggest that the increased GnIH expression could be a consequence of the reduced food intake, and would not be a direct response to HT.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume162
Issue number3
DOIs
Publication statusPublished - Jul 1 2012

Fingerprint

RNA Precursors
Gonadotropins
Eating
Hot Temperature
Hormones
Temperature
Metabolites
Chickens
Paraventricular Hypothalamic Nucleus
Plasmas
Appetite Regulation
Diencephalon
Uric Acid
Neuropeptides
Temperature control
Hypothalamus
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Molecular Biology

Cite this

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title = "Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks",
abstract = "The regulation of food intake in chickens (. Gallus gallus domesticus) represents a complex homeostatic mechanism involving multiple levels of control, and regulation during high ambient temperatures (HT) is poorly understood. In this study, we examined hypothalamic mRNA expression of gonadotropin-inhibitory hormone (GnIH) to understand the effect of HT on an orexigenic neuropeptide. We examined the effects of HT (35. °C ambient temperature for 1, 24 or 48. h) on 14-day old chicks. HT significantly increased rectal temperature and suppressed food intake, and also influenced plasma metabolites. The expression of GnIH precursor mRNA in the diencephalon was significantly increased in chicks at 24-and 48. h of HT when food intake was suppressed significantly, whilst no change was observed for GnIH precursor mRNA and food intake at 1. h of HT. . In situ hybridization and immunocytochemistry further revealed the cellular localization of chicken GnIH precursor mRNA and its peptide in the paraventricular nucleus (PVN) in the chick hypothalamus. We examined plasma metabolites in chicks exposed to HT for 1 or 48. h and found that triacylglycerol concentration was significantly higher in HT than control chicks at 1. h. Total protein, uric acid and calcium were significantly lower in HT chicks than control chicks at 48. h. These results indicate that not only a reduction in food intake and alteration in plasma metabolites but also the PVN-specific expression of GnIH, an orexigenic agent, may be induced by HT. The reduced food intake at the same time as GnIH expression was increased during HT suggests that HT-induced GnIH expression may oppose HT-induced feeding suppression, rather than promote it. We suggest that the increased GnIH expression could be a consequence of the reduced food intake, and would not be a direct response to HT.",
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T1 - Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks

AU - Surchowdhury, Vishwajit

AU - Tomonaga, Shozo

AU - Nishimura, Shotaro

AU - Tabata, Shoji

AU - Cockrem, John F.

AU - Tsutsui, Kazuyoshi

AU - Furuse, Mitsuhiro

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N2 - The regulation of food intake in chickens (. Gallus gallus domesticus) represents a complex homeostatic mechanism involving multiple levels of control, and regulation during high ambient temperatures (HT) is poorly understood. In this study, we examined hypothalamic mRNA expression of gonadotropin-inhibitory hormone (GnIH) to understand the effect of HT on an orexigenic neuropeptide. We examined the effects of HT (35. °C ambient temperature for 1, 24 or 48. h) on 14-day old chicks. HT significantly increased rectal temperature and suppressed food intake, and also influenced plasma metabolites. The expression of GnIH precursor mRNA in the diencephalon was significantly increased in chicks at 24-and 48. h of HT when food intake was suppressed significantly, whilst no change was observed for GnIH precursor mRNA and food intake at 1. h of HT. . In situ hybridization and immunocytochemistry further revealed the cellular localization of chicken GnIH precursor mRNA and its peptide in the paraventricular nucleus (PVN) in the chick hypothalamus. We examined plasma metabolites in chicks exposed to HT for 1 or 48. h and found that triacylglycerol concentration was significantly higher in HT than control chicks at 1. h. Total protein, uric acid and calcium were significantly lower in HT chicks than control chicks at 48. h. These results indicate that not only a reduction in food intake and alteration in plasma metabolites but also the PVN-specific expression of GnIH, an orexigenic agent, may be induced by HT. The reduced food intake at the same time as GnIH expression was increased during HT suggests that HT-induced GnIH expression may oppose HT-induced feeding suppression, rather than promote it. We suggest that the increased GnIH expression could be a consequence of the reduced food intake, and would not be a direct response to HT.

AB - The regulation of food intake in chickens (. Gallus gallus domesticus) represents a complex homeostatic mechanism involving multiple levels of control, and regulation during high ambient temperatures (HT) is poorly understood. In this study, we examined hypothalamic mRNA expression of gonadotropin-inhibitory hormone (GnIH) to understand the effect of HT on an orexigenic neuropeptide. We examined the effects of HT (35. °C ambient temperature for 1, 24 or 48. h) on 14-day old chicks. HT significantly increased rectal temperature and suppressed food intake, and also influenced plasma metabolites. The expression of GnIH precursor mRNA in the diencephalon was significantly increased in chicks at 24-and 48. h of HT when food intake was suppressed significantly, whilst no change was observed for GnIH precursor mRNA and food intake at 1. h of HT. . In situ hybridization and immunocytochemistry further revealed the cellular localization of chicken GnIH precursor mRNA and its peptide in the paraventricular nucleus (PVN) in the chick hypothalamus. We examined plasma metabolites in chicks exposed to HT for 1 or 48. h and found that triacylglycerol concentration was significantly higher in HT than control chicks at 1. h. Total protein, uric acid and calcium were significantly lower in HT chicks than control chicks at 48. h. These results indicate that not only a reduction in food intake and alteration in plasma metabolites but also the PVN-specific expression of GnIH, an orexigenic agent, may be induced by HT. The reduced food intake at the same time as GnIH expression was increased during HT suggests that HT-induced GnIH expression may oppose HT-induced feeding suppression, rather than promote it. We suggest that the increased GnIH expression could be a consequence of the reduced food intake, and would not be a direct response to HT.

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