Hypotonic stress-induced NO production in endothelium depends on endogenous ATP

Chiwaka Kimura, Tetsuya Koyama, Masahiro Oike, Yushi Ito

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The mechanism by which mechanical stress induces nitric oxide (NO) synthesis in endothelium is still controversial. Hypotonic stress (HTS, -20%) induced ATP release, which evoked Ca2+ transients in bovine aortic endothelial cells (BAEC). HTS also induced NO synthesis, assessed by DAF-2 fluorescence, which was suppressed by inhibiting endogenous ATP-induced Ca2+ transients with suramin or neomycin. Exogenously applied ATP mimicked these responses. Pretreatment with wortmannin did not affect DAF-2 fluorescence, suggesting that Akt phosphorylation was not involved in HTS-induced NO synthesis. These results indicate that endogenous ATP plays a central role in HTS-induced NO synthesis in BAEC. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)736-740
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume274
Issue number3
DOIs
Publication statusPublished - Aug 11 2000

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Osmotic Pressure
Endothelium
Nitric Oxide
Adenosine Triphosphate
Endothelial cells
Endothelial Cells
Fluorescence
Suramin
Mechanical Stress
Phosphorylation
Neomycin

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hypotonic stress-induced NO production in endothelium depends on endogenous ATP. / Kimura, Chiwaka; Koyama, Tetsuya; Oike, Masahiro; Ito, Yushi.

In: Biochemical and Biophysical Research Communications, Vol. 274, No. 3, 11.08.2000, p. 736-740.

Research output: Contribution to journalArticle

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