Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons

Nobuaki Egashira, Katsunori Iwasaki, Motoki Ishibashi, Izzetin Hatip-Al-Khatib, Benjamin Wolozin, Kenichi Mishima, Keiichi Irie, Michihiro Fujiwara

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Abstract

We investigated the effect of hypoxia on β-amyloid (Aβ)-induced apoptosis in rat cultured hippocampal neurons. Aβ (25 μM for 48 h) decreased the number of neuronal cells and increased the number of TUNEL-positive cells. Hypoxia (6 h) also decreased the number of neuronal cells, but did not increase the number of TUNEL-positive cells. Moreover, combined treatment with both Aβ and hypoxia (Aβ/hypoxia) significantly enhanced in decrease in the number of neuronal cells and the increase in the number of TUNEL-positive cells. Z-Asp-CH2-DCB, an inhibitor of interleukin-1β-converting enzyme (ICE), or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), a non-N-methyl-D-aspartate (non-NMDA) receptor antagonist, decreased the number of TUNEL-positive cells with Aβ/hypoxia. These findings suggest that ischemia or hypoxia is an important factor that facilitates the symptoms of Alzheimer's disease and that non-NMDA receptors are involved in the induction of apoptosis in patients suffering from both cerebrovascular disease and Alzheimer's disease.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalJapanese Journal of Pharmacology
Volume90
Issue number4
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology

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    Egashira, N., Iwasaki, K., Ishibashi, M., Hatip-Al-Khatib, I., Wolozin, B., Mishima, K., Irie, K., & Fujiwara, M. (2002). Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons. Japanese Journal of Pharmacology, 90(4), 321-327. https://doi.org/10.1254/jjp.90.321