Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons

Nobuaki Egashira, Katsunori Iwasaki, Motoki Ishibashi, Izzetin Hatip-Al-Khatib, Benjamin Wolozin, Kenichi Mishima, Keiichi Irie, Michihiro Fujiwara

Research output: Contribution to journalArticle

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Abstract

We investigated the effect of hypoxia on β-amyloid (Aβ)-induced apoptosis in rat cultured hippocampal neurons. Aβ (25 μM for 48 h) decreased the number of neuronal cells and increased the number of TUNEL-positive cells. Hypoxia (6 h) also decreased the number of neuronal cells, but did not increase the number of TUNEL-positive cells. Moreover, combined treatment with both Aβ and hypoxia (Aβ/hypoxia) significantly enhanced in decrease in the number of neuronal cells and the increase in the number of TUNEL-positive cells. Z-Asp-CH2-DCB, an inhibitor of interleukin-1β-converting enzyme (ICE), or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), a non-N-methyl-D-aspartate (non-NMDA) receptor antagonist, decreased the number of TUNEL-positive cells with Aβ/hypoxia. These findings suggest that ischemia or hypoxia is an important factor that facilitates the symptoms of Alzheimer's disease and that non-NMDA receptors are involved in the induction of apoptosis in patients suffering from both cerebrovascular disease and Alzheimer's disease.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalJapanese Journal of Pharmacology
Volume90
Issue number4
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

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Amyloid
In Situ Nick-End Labeling
Apoptosis
Neurons
D-Aspartic Acid
Cell Count
Alzheimer Disease
6-Cyano-7-nitroquinoxaline-2,3-dione
Cerebrovascular Disorders
Caspase 1
Ischemia
Hypoxia
aspartic acid receptor
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Egashira, N., Iwasaki, K., Ishibashi, M., Hatip-Al-Khatib, I., Wolozin, B., Mishima, K., ... Fujiwara, M. (2002). Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons. Japanese Journal of Pharmacology, 90(4), 321-327. https://doi.org/10.1254/jjp.90.321

Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons. / Egashira, Nobuaki; Iwasaki, Katsunori; Ishibashi, Motoki; Hatip-Al-Khatib, Izzetin; Wolozin, Benjamin; Mishima, Kenichi; Irie, Keiichi; Fujiwara, Michihiro.

In: Japanese Journal of Pharmacology, Vol. 90, No. 4, 01.12.2002, p. 321-327.

Research output: Contribution to journalArticle

Egashira, N, Iwasaki, K, Ishibashi, M, Hatip-Al-Khatib, I, Wolozin, B, Mishima, K, Irie, K & Fujiwara, M 2002, 'Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons', Japanese Journal of Pharmacology, vol. 90, no. 4, pp. 321-327. https://doi.org/10.1254/jjp.90.321
Egashira, Nobuaki ; Iwasaki, Katsunori ; Ishibashi, Motoki ; Hatip-Al-Khatib, Izzetin ; Wolozin, Benjamin ; Mishima, Kenichi ; Irie, Keiichi ; Fujiwara, Michihiro. / Hypoxia enhances β-amyloid-induced apoptosis in rat cultured hippocampal neurons. In: Japanese Journal of Pharmacology. 2002 ; Vol. 90, No. 4. pp. 321-327.
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