TY - JOUR
T1 - Id-1 is not expressed in the luminal epithelial cells of mammary glands
AU - Uehara, Norihisa
AU - Chou, Yu Chien
AU - Galvez, Jose J.
AU - de-Candia, Paola
AU - Cardiff, Robert D.
AU - Benezra, Robert
AU - Shyamala, Gopalan
N1 - Funding Information:
These studies were supported by a grant from NIH (CA 66541) to GS, a grant from State of California Breast Cancer Research program (5Jb-0014) to RDC, and a grant from Breast Cancer Research Foundation to RB. NU was supported by institution training grant DAMD17-00-1-0224. PdC was supported by a fellowship from the Italian American Cancer Foundation.
Publisher Copyright:
© 2003 Uehara et al..
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Background: The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry. Methods: Extracts of whole mammary glands from wild type and Id-1 null mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1. Results: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells. Conclusion: Id-1 is not expressed in the luminal epithelial cells of mammary glands.
AB - Background: The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry. Methods: Extracts of whole mammary glands from wild type and Id-1 null mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1. Results: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells. Conclusion: Id-1 is not expressed in the luminal epithelial cells of mammary glands.
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U2 - 10.1186/bcr560
DO - 10.1186/bcr560
M3 - Article
C2 - 12631395
AN - SCOPUS:0038700564
SN - 1465-5411
VL - 5
JO - Breast Cancer Research
JF - Breast Cancer Research
IS - 2
M1 - R25
ER -
