Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization

Yasushi Shibata, Takashi Iwamatsu, Yuichi Oba, Daisuke Kobayashi, Minoru Tanaka, Yoshitaka Nagahama, Norio Suzuki, Michiyasu Yoshikuni

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Chorion hardening is triggered by the contents of cortical alveoli that are released upon fertilization of medaka (Oryzias latipes) eggs. We purified the chorion hardening-inducing activity as a single protein from the exudate of cortical alveoli of medaka eggs. This activity was co-purified with proteolytic activity of the chorion protein ZI-1,2. Based on the amino acid sequence of purified protein, we cloned the cDNA of this protein from a medaka ovarian cDNA library. Sequence analyses revealed typical sequence features, a zinc-binding motif and a methionine turn motif, of the astacin metalloproteinase family. We termed this protein 'alveolin.' Alveolin has a molecular mass of 21.5 kDa deduced by the amino acid sequence and neutral optimal pH range. Alveolin hydrolyzes ZI-1,2. Alveolin activity was strongly inhibited by metal-chelating agents but not by various proteinase inhibitors. To our knowledge, this is the first description of the isolation and identification of the chorion hardening-inducing factor from cortical alveoli exudate of teleost eggs.

Original languageEnglish
Pages (from-to)8349-8354
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number12
DOIs
Publication statusPublished - Mar 24 2000
Externally publishedYes

Fingerprint

Oryzias
Chorion
Cloning
Metalloproteases
Fertilization
Eggs
Hardening
Organism Cloning
Complementary DNA
Exudates and Transudates
Amino Acid Sequence
Proteins
Amino Acids
Molecular mass
Chelating Agents
Gene Library
Methionine
Sequence Analysis
Zinc
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization. / Shibata, Yasushi; Iwamatsu, Takashi; Oba, Yuichi; Kobayashi, Daisuke; Tanaka, Minoru; Nagahama, Yoshitaka; Suzuki, Norio; Yoshikuni, Michiyasu.

In: Journal of Biological Chemistry, Vol. 275, No. 12, 24.03.2000, p. 8349-8354.

Research output: Contribution to journalArticle

Shibata, Yasushi ; Iwamatsu, Takashi ; Oba, Yuichi ; Kobayashi, Daisuke ; Tanaka, Minoru ; Nagahama, Yoshitaka ; Suzuki, Norio ; Yoshikuni, Michiyasu. / Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 12. pp. 8349-8354.
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