Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)

Masato Matsushita, Tomoki Yamadori, Seishi Kato, Yoshihiro Takemoto, Jouji Inazawa, Yoshihiro Baba, Shoji Hashimoto, Shingo Sekine, Shigeyuki Arai, Toshio Kunikata, Masashi Kurimoto, Tadamitsu Kishimoto, Satoshi Tsukada

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Protein interaction cloning method was used to identify a novel molecule, Sab, which binds to the SH3 domain of Bruton's tyrosine kinase (Btk), the deficient cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia and murine X-linked immunodeficiency. Immunoprecipitation using the anti-Sab antibody identified the protein product of the gene as a 70 kDa molecule. While Sab does not have a proline-rich sequence, it was shown to bind to Btk through the commonly conserved structure among SH3 domains. Remarkably, Sab exhibited a high preference for binding to Btk rather than to other cytoplasmic tyrosine kinases, which suggests a unique role of Sab in the Btk signal transduction pathway.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume245
Issue number2
DOIs
Publication statusPublished - Apr 17 1998
Externally publishedYes

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src Homology Domains
Carrier Proteins
Protein-Tyrosine Kinases
Signal transduction
Molecules
Cloning
Immunoprecipitation
Proline
Organism Cloning
Anti-Idiotypic Antibodies
Signal Transduction
Proteins
Agammaglobulinaemia tyrosine kinase
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk). / Matsushita, Masato; Yamadori, Tomoki; Kato, Seishi; Takemoto, Yoshihiro; Inazawa, Jouji; Baba, Yoshihiro; Hashimoto, Shoji; Sekine, Shingo; Arai, Shigeyuki; Kunikata, Toshio; Kurimoto, Masashi; Kishimoto, Tadamitsu; Tsukada, Satoshi.

In: Biochemical and Biophysical Research Communications, Vol. 245, No. 2, 17.04.1998, p. 337-343.

Research output: Contribution to journalArticle

Matsushita, M, Yamadori, T, Kato, S, Takemoto, Y, Inazawa, J, Baba, Y, Hashimoto, S, Sekine, S, Arai, S, Kunikata, T, Kurimoto, M, Kishimoto, T & Tsukada, S 1998, 'Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)', Biochemical and Biophysical Research Communications, vol. 245, no. 2, pp. 337-343. https://doi.org/10.1006/bbrc.1998.8420
Matsushita, Masato ; Yamadori, Tomoki ; Kato, Seishi ; Takemoto, Yoshihiro ; Inazawa, Jouji ; Baba, Yoshihiro ; Hashimoto, Shoji ; Sekine, Shingo ; Arai, Shigeyuki ; Kunikata, Toshio ; Kurimoto, Masashi ; Kishimoto, Tadamitsu ; Tsukada, Satoshi. / Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk). In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 245, No. 2. pp. 337-343.
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