Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha

Phatthanon Prasitchoke, Yoshinobu Kaneko, Takeshi Bamba, Eiichiro Fukusaki, Akio Kobayashi, Satoshi Harashima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To understand the biosynthetic network of fatty acids in the methylotrophic yeast Hansenula polymorpha, which is able to produce poly-unsaturated fatty acids, we have attempted to identify genes encoding fatty acid elongase. Here we have characterized HpELO1, a fatty acid elongase gene encoding a 319-amino-acid protein containing five predicted membrane-spanning regions that is conserved throughout the yeast Elo protein family. Phylogenetic analysis of the deduced amino acid sequence suggests that HpELO1 is an ortholog of the Saccharomyces cerevisiae ELO3 gene that is involved in the elongation of very long-chain fatty acids (VLCFAs). In the fatty acid profile of the Hpelo1Δ disruptant by gas chromatography/mass spectrometry, the amount of C24:0 and C26:0 decreased to undetectable levels, whereas there was a large accumulation of C22:0, suggesting that the HpELO1 is involved in the elongation of VLCFAs and is essential for the production of C24:0. Expression of HpELO1 suppressed the lethality of the S. cerevisiae elo2Δ elo3Δ double disruptant and recovered the synthesis of VLCFAs. Similar to the S. cerevisiae elo3Δ strain, the Hpelo1Δ disruptant exhibited the extraordinary growth sensitivity to fumonisin B1, a ceramide synthase inhibitor. Furthermore, cells of the Hpelo1Δ disruptant were more sensitive to Zymolyase and more flocculent than the wild-type cells, clumping together and falling rapidly out of suspension, suggesting that the Hpelo1Δ mutation causes changes in cell wall composition and structure.

Original languageEnglish
Pages (from-to)16-25
Number of pages10
JournalGene
Volume391
Issue number1-2
DOIs
Publication statusPublished - Apr 15 2007
Externally publishedYes

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Pichia
Fatty Acids
Yeasts
Saccharomyces cerevisiae
Genes
Essential Fatty Acids
Fungal Proteins
Protein Sequence Analysis
Unsaturated Fatty Acids
Gas Chromatography-Mass Spectrometry
Cell Wall
Suspensions
Amino Acids
Mutation
Membranes
fatty acid elongases
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha. / Prasitchoke, Phatthanon; Kaneko, Yoshinobu; Bamba, Takeshi; Fukusaki, Eiichiro; Kobayashi, Akio; Harashima, Satoshi.

In: Gene, Vol. 391, No. 1-2, 15.04.2007, p. 16-25.

Research output: Contribution to journalArticle

Prasitchoke, Phatthanon ; Kaneko, Yoshinobu ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Kobayashi, Akio ; Harashima, Satoshi. / Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha. In: Gene. 2007 ; Vol. 391, No. 1-2. pp. 16-25.
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