Identification and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. Isolated from shrimp culture pond sediments in thua Thien Hue-Viet Nam

Ngo Thi Tuong Chau, Nguyen Xuan Hieu, Le Thi Nam Thuan, Matsumoto Masaru, Ikuo Miyajima

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7 Citations (Scopus)

Abstract

The shrimp diseases in Thua Thien Hue province have mainly accounted for vibriosis caused by Vibrio spp. The addition of substantial amounts of antibiotics is still the method of choice for control the proliferation of pathogenic Vibrio spp. However the abuse of antibiotic agents has led to the emergence of antibiotic-resistant bacteria which may result to resistance transfer to pathogenic bacteria and accumulation of antibiotics in shrimp products. Therefore, developing an alternative strategy to control infections is urgently needed. Following this goal, in the present study, indigenous actinomycetes were isolated from shrimp pond sediments in Thua Thien Hue and strain Al was screened for their activity against two pathogenic strains Vibrio sp. V7 and V10 derived from diseased shrimps from culture ponds in Thua Thien Hue. Based on the cell morphology observed with a JEOL 5410 LV scanning electron microscope and 16S rRNA nucleotide sequence used to search the GenBank database with the BLAST system, strain Al was belong to Streptomyces genus and identified as Streptomyces sp. Al. The 16S rRNA sequence of Streptomyces sp. Al has been deposited in the DDBJ/EMBL/GenBank database with the accession number HM854225. Besides, Streptomyces sp. Al also showed ability to produce siderophore to acquire iron and extracellular enzymes to decompose organic compounds such as starch, protein and cellulose. Otherwise, Streptomyces sp. Al was non-pathogenic to shrimp. Therefore, Streptomyces sp. Al can be considered as not only a candidate for control the shrimp diseases but also a potential probiont for srdimp culture.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume56
Issue number1
Publication statusPublished - Feb 1 2011

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shrimp culture
Vibrio
Actinobacteria
Vietnam
Streptomyces
shrimp
sediments
Nucleic Acid Databases
antibiotics
Anti-Bacterial Agents
ribosomal RNA
Databases
Bacteria
pond culture
vibriosis
Siderophores
siderophores
bacteria
Infection Control
scanning electron microscopes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Identification and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. Isolated from shrimp culture pond sediments in thua Thien Hue-Viet Nam",
abstract = "The shrimp diseases in Thua Thien Hue province have mainly accounted for vibriosis caused by Vibrio spp. The addition of substantial amounts of antibiotics is still the method of choice for control the proliferation of pathogenic Vibrio spp. However the abuse of antibiotic agents has led to the emergence of antibiotic-resistant bacteria which may result to resistance transfer to pathogenic bacteria and accumulation of antibiotics in shrimp products. Therefore, developing an alternative strategy to control infections is urgently needed. Following this goal, in the present study, indigenous actinomycetes were isolated from shrimp pond sediments in Thua Thien Hue and strain Al was screened for their activity against two pathogenic strains Vibrio sp. V7 and V10 derived from diseased shrimps from culture ponds in Thua Thien Hue. Based on the cell morphology observed with a JEOL 5410 LV scanning electron microscope and 16S rRNA nucleotide sequence used to search the GenBank database with the BLAST system, strain Al was belong to Streptomyces genus and identified as Streptomyces sp. Al. The 16S rRNA sequence of Streptomyces sp. Al has been deposited in the DDBJ/EMBL/GenBank database with the accession number HM854225. Besides, Streptomyces sp. Al also showed ability to produce siderophore to acquire iron and extracellular enzymes to decompose organic compounds such as starch, protein and cellulose. Otherwise, Streptomyces sp. Al was non-pathogenic to shrimp. Therefore, Streptomyces sp. Al can be considered as not only a candidate for control the shrimp diseases but also a potential probiont for srdimp culture.",
author = "Chau, {Ngo Thi Tuong} and Hieu, {Nguyen Xuan} and Thuan, {Le Thi Nam} and Matsumoto Masaru and Ikuo Miyajima",
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T1 - Identification and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. Isolated from shrimp culture pond sediments in thua Thien Hue-Viet Nam

AU - Chau, Ngo Thi Tuong

AU - Hieu, Nguyen Xuan

AU - Thuan, Le Thi Nam

AU - Masaru, Matsumoto

AU - Miyajima, Ikuo

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