Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by lactococcus sp. strain QU 12

Naruhiko Sawa, Takeshi Zendo, Junko Kiyofuji, Koji Fujita, Kohei Himeno, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Lactococcus sp. strain QU 12, which was isolated from cheese, produced a novel cyclic bacteriocin termed lactocyclicin Q. By using cation-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high-performance liquid chromatography, lactocyclicin Q was purified from culture supernatant, and its molecular mass was determined to be 6,062.8 Da by mass spectrometry. Lactocyclicin Q has been characterized by its unique antimicrobial spectrum, high level of protease resistance, and heat stability compared to other reported bacteriocins of lactic acid bacteria. The amino acid sequence of lactocyclicin Q was determined chemically, and this compound is composed of 61 amino acid residues that have a cyclic structure with linkage between the N and C termini by a peptide bond. It showed no homology to any other antimicrobial peptide, including cyclic bacteriocins. On the basis of the amino acid sequences obtained, the sequence of the gene encoding the prepeptide lactocyclicin Q was obtained. This is the first report of a cyclic bacteriocin purified from a strain belonging to the genus Lactococcus.

Original languageEnglish
Pages (from-to)1552-1558
Number of pages7
JournalApplied and environmental microbiology
Volume75
Issue number6
DOIs
Publication statusPublished - Mar 1 2009

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Lactococcus
Bacteriocins
bacteriocins
amino acid
peptide
chromatography
Chromatography
Amino Acid Sequence
amino acid sequences
homology
Cyclic Peptides
heat stability
liquid chromatography
reversed-phase liquid chromatography
antimicrobial peptides
ion exchange
Cheese
Reverse-Phase Chromatography
mass spectrometry
Hydrophobic and Hydrophilic Interactions

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by lactococcus sp. strain QU 12. / Sawa, Naruhiko; Zendo, Takeshi; Kiyofuji, Junko; Fujita, Koji; Himeno, Kohei; Nakayama, Jiro; Sonomoto, Kenji.

In: Applied and environmental microbiology, Vol. 75, No. 6, 01.03.2009, p. 1552-1558.

Research output: Contribution to journalArticle

Sawa, Naruhiko ; Zendo, Takeshi ; Kiyofuji, Junko ; Fujita, Koji ; Himeno, Kohei ; Nakayama, Jiro ; Sonomoto, Kenji. / Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by lactococcus sp. strain QU 12. In: Applied and environmental microbiology. 2009 ; Vol. 75, No. 6. pp. 1552-1558.
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AU - Himeno, Kohei

AU - Nakayama, Jiro

AU - Sonomoto, Kenji

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