Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin

Kunihiko Tamaki, Nami Yasaka, Chung Hsing Chang, Naoto Ohtake, Atsushi Saitoh, Koichiro Nakamura, Masutaka Furue

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Using immunofluorescence on dermal sheets of mouse ear, we identified novel Thy-1+ dermal dendritic cells in murine skin. These cells are resistant to topical corticosteroid treatment. The number of these dermal Thy-1+ dendritic cells is not altered with aging of the mice or with 3 d of culture. Dermal Thy-1+ dendritic cells have melanosomes in their cytoplasm; hence, phagocytic activity is likely to be present. Double immunofluorescence revealed that most of these dermal Thy-1+ dendritic cells express CD45. Furthermore, conventional immunofluorescence and confocal laser scanning microscopic findings showed that most of these Thy-1+ dermal dendritic cells express γδ and Vγ3 T-cell receptors. The relationship between these dermal Thy-1+ dendritic cells and dendritic epidermal T cells is discussed.

Original languageEnglish
Pages (from-to)571-575
Number of pages5
JournalJournal of Investigative Dermatology
Volume106
Issue number3
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Bearings (structural)
Thy-1 Antigens
Dendritic Cells
Skin
Langerhans Cells
Fluorescent Antibody Technique
Melanosomes
T-cells
T-Cell Antigen Receptor
Ear
Adrenal Cortex Hormones
Cytoplasm
Lasers
Aging of materials
Scanning
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin. / Tamaki, Kunihiko; Yasaka, Nami; Chang, Chung Hsing; Ohtake, Naoto; Saitoh, Atsushi; Nakamura, Koichiro; Furue, Masutaka.

In: Journal of Investigative Dermatology, Vol. 106, No. 3, 01.01.1996, p. 571-575.

Research output: Contribution to journalArticle

Tamaki, Kunihiko ; Yasaka, Nami ; Chang, Chung Hsing ; Ohtake, Naoto ; Saitoh, Atsushi ; Nakamura, Koichiro ; Furue, Masutaka. / Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin. In: Journal of Investigative Dermatology. 1996 ; Vol. 106, No. 3. pp. 571-575.
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