Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98

N. Sawa, S. Koga, K. Okamura, N. Ishibashi, Takeshi Zendo, Kenji Sonomoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim: To characterize novel multiple bacteriocins produced by Lactobacillus sakei D98. Methods and Results: Lactobacillus sakei D98 isolated from Shubo (rice malt) produced at least three bacteriocins. Using three purification steps, three novel antimicrobial peptides termed sakacin D98a, sakacin D98b and sakacin D98c were purified from the culture supernatant. Amino acid and DNA sequencing analysis revealed that the sakacins D98a, D98b and D98c are novel class IIa-like or class IId bacteriocins. In particular, sakacin D98b has a variant pediocin-box sequence, YANGVXC (with Ala instead of Gly), and a different location for the disulfide bridge (Cys11 and Cys18) from that found in other class IIa bacteriocins. Conclusions: Three novel bacteriocins were identified from Lactobacillus sakei D98. Their antimicrobial spectra and intensities indicate that these sakacins would have different modes of action. In addition, sakacin D98b showed low inhibitory activity against Listeria, probably due to the differences in amino acids and position of the disulfide bridge compared with the other class IIa bacteriocins. Significance and Impact of Study: Sakacins D98a and D98c are novel bacteriocins belonging to class IId bacteriocins. On the other hand, sakacin D98b, a class IIa-like bacteriocin, has a unique internal structure and activity spectrum.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalJournal of Applied Microbiology
Volume115
Issue number1
DOIs
Publication statusPublished - Jul 1 2013

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Bacteriocins
Disulfides
Lactobacillus sakei
Listeria
Protein Sequence Analysis
DNA Sequence Analysis
Amino Acids
Peptides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98. / Sawa, N.; Koga, S.; Okamura, K.; Ishibashi, N.; Zendo, Takeshi; Sonomoto, Kenji.

In: Journal of Applied Microbiology, Vol. 115, No. 1, 01.07.2013, p. 61-69.

Research output: Contribution to journalArticle

Sawa, N. ; Koga, S. ; Okamura, K. ; Ishibashi, N. ; Zendo, Takeshi ; Sonomoto, Kenji. / Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98. In: Journal of Applied Microbiology. 2013 ; Vol. 115, No. 1. pp. 61-69.
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