Identification and characterization of pseudomonas sp. P9 antagonistic to pathogenic vibrio spp. Isolated from shrimp culture pond in Thua Thien Hue-Viet Nam

Ngo Thi Tuong Chau, Pham Huu Quang, Pham Thi Ngoc Lan, Matsumoto Masaru, Ikuo Miyajima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

From shrimp culture ponds in Thua Thien Hue, isolates were obtained on PSA plates and screened for their activity against two pathogenic strains Vibrio sp. V7 and V10 isolated from diseased shrimps in culture ponds in Thua Thien Hue using the agar well-diffusion method. Among of the isolates, only strain P9 showed antagonistic activity against Vibrio sp. V7 and V10. Based on the cell morphology observed with a JEOL 5410 LV scanning electron microscope, biochemical reactions tested with GN card, and 16S rRNA nucleotide sequence used to search the GenBank database with the BLAST system, strain P9 was identified as Pseudomonas sp. P9. The 16S rRNA sequence of Pseudomonas sp. P9 has been deposited in the DDBJ/ EMBIVGenBank database with the accession number HM854226. In addition, Pseudomonas sp. P9 also showed the ability to produce siderophore to acquire iron and extracellular enzymes to decompose organic compounds such as starch, protein and cellulose. Otherwise Pseudomonas sp. P9 was non-pathogenic to shrimp. Growth as well as production of the antagonistic component in TSBS of Pseudomonas sp. P9 showed over a wide range of pH, salinities and temperatures. Therefore, Pseudomonas sp. P9 can be considered as not only a candidate for control the shrimp disease but also a potential probiont for shrimp culture.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume56
Issue number1
Publication statusPublished - Feb 1 2011

Fingerprint

shrimp culture
Vibrio
Vietnam
Pseudomonas
Nucleic Acid Databases
shrimp
ribosomal RNA
Databases
Siderophores
siderophores
Salinity
chemical reactions
scanning electron microscopes
organic compounds
Cellulose
Starch
Agar
cellulose
Iron
agar

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Identification and characterization of pseudomonas sp. P9 antagonistic to pathogenic vibrio spp. Isolated from shrimp culture pond in Thua Thien Hue-Viet Nam",
abstract = "From shrimp culture ponds in Thua Thien Hue, isolates were obtained on PSA plates and screened for their activity against two pathogenic strains Vibrio sp. V7 and V10 isolated from diseased shrimps in culture ponds in Thua Thien Hue using the agar well-diffusion method. Among of the isolates, only strain P9 showed antagonistic activity against Vibrio sp. V7 and V10. Based on the cell morphology observed with a JEOL 5410 LV scanning electron microscope, biochemical reactions tested with GN card, and 16S rRNA nucleotide sequence used to search the GenBank database with the BLAST system, strain P9 was identified as Pseudomonas sp. P9. The 16S rRNA sequence of Pseudomonas sp. P9 has been deposited in the DDBJ/ EMBIVGenBank database with the accession number HM854226. In addition, Pseudomonas sp. P9 also showed the ability to produce siderophore to acquire iron and extracellular enzymes to decompose organic compounds such as starch, protein and cellulose. Otherwise Pseudomonas sp. P9 was non-pathogenic to shrimp. Growth as well as production of the antagonistic component in TSBS of Pseudomonas sp. P9 showed over a wide range of pH, salinities and temperatures. Therefore, Pseudomonas sp. P9 can be considered as not only a candidate for control the shrimp disease but also a potential probiont for shrimp culture.",
author = "Chau, {Ngo Thi Tuong} and Quang, {Pham Huu} and Lan, {Pham Thi Ngoc} and Matsumoto Masaru and Ikuo Miyajima",
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T1 - Identification and characterization of pseudomonas sp. P9 antagonistic to pathogenic vibrio spp. Isolated from shrimp culture pond in Thua Thien Hue-Viet Nam

AU - Chau, Ngo Thi Tuong

AU - Quang, Pham Huu

AU - Lan, Pham Thi Ngoc

AU - Masaru, Matsumoto

AU - Miyajima, Ikuo

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N2 - From shrimp culture ponds in Thua Thien Hue, isolates were obtained on PSA plates and screened for their activity against two pathogenic strains Vibrio sp. V7 and V10 isolated from diseased shrimps in culture ponds in Thua Thien Hue using the agar well-diffusion method. Among of the isolates, only strain P9 showed antagonistic activity against Vibrio sp. V7 and V10. Based on the cell morphology observed with a JEOL 5410 LV scanning electron microscope, biochemical reactions tested with GN card, and 16S rRNA nucleotide sequence used to search the GenBank database with the BLAST system, strain P9 was identified as Pseudomonas sp. P9. The 16S rRNA sequence of Pseudomonas sp. P9 has been deposited in the DDBJ/ EMBIVGenBank database with the accession number HM854226. In addition, Pseudomonas sp. P9 also showed the ability to produce siderophore to acquire iron and extracellular enzymes to decompose organic compounds such as starch, protein and cellulose. Otherwise Pseudomonas sp. P9 was non-pathogenic to shrimp. Growth as well as production of the antagonistic component in TSBS of Pseudomonas sp. P9 showed over a wide range of pH, salinities and temperatures. Therefore, Pseudomonas sp. P9 can be considered as not only a candidate for control the shrimp disease but also a potential probiont for shrimp culture.

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