Identification and characterization of two novel classes of small RNAs in the mouse germline: Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes

Toshiaki Watanabe, Atsushi Takeda, Tomoyuki Tsukiyama, Kazuyuki Mise, Tetsuro Okuno, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai

Research output: Contribution to journalArticle

425 Citations (Scopus)

Abstract

Small RNAs ranging in size between 18 and 30 nucleotides (nt) are found in many organisms including yeasts, plants, and animals. Small RNAs are involved in the regulation of gene expression through translational repression, mRNA degradation, and chromatin modification. In mammals, microRNAs (miRNAs) are the only small RNAs that have been well characterized. Here, we have identified two novel classes of small RNAs in the mouse germline. One class consists of ∼20- to 24-nt small interfering RNAs (siRNAs) from mouse oocytes, which are derived from retroelements including LINE, SINE, and LTR retrotransposons. Addition of retrotransposon-derived sequences to the 3′ untranslated region (UTR) of a reporter mRNA destabilizes the mRNA significantly when injected into full-grown oocytes. These results suggest that retrotransposons are suppressed through the RNAi pathway in mouse oocytes. The other novel class of small RNAs is 26- to 30-nt germline small RNAs (gsRNAs) from testes. gsRNAs are expressed during spermatogenesis in a developmentally regulated manner, are mapped to the genome in clusters, and have strong strand bias. These features are reminiscent of Tetrahymena ∼23- to 24-nt small RNAs and Caenorhabditis elegans X-cluster small RNAs. A conserved novel small RNA pathway may be present in diverse animals.

Original languageEnglish
Pages (from-to)1732-1743
Number of pages12
JournalGenes and Development
Volume20
Issue number13
DOIs
Publication statusPublished - Jul 1 2006
Externally publishedYes

Fingerprint

Retroelements
Small Interfering RNA
Oocytes
Testis
RNA
Nucleotides
Short Interspersed Nucleotide Elements
Tetrahymena
Messenger RNA
RNA Stability
Caenorhabditis elegans
Gene Expression Regulation
3' Untranslated Regions
Spermatogenesis
RNA Interference
MicroRNAs
Chromatin
Mammals
Yeasts
Genome

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Identification and characterization of two novel classes of small RNAs in the mouse germline : Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. / Watanabe, Toshiaki; Takeda, Atsushi; Tsukiyama, Tomoyuki; Mise, Kazuyuki; Okuno, Tetsuro; Sasaki, Hiroyuki; Minami, Naojiro; Imai, Hiroshi.

In: Genes and Development, Vol. 20, No. 13, 01.07.2006, p. 1732-1743.

Research output: Contribution to journalArticle

Watanabe, Toshiaki ; Takeda, Atsushi ; Tsukiyama, Tomoyuki ; Mise, Kazuyuki ; Okuno, Tetsuro ; Sasaki, Hiroyuki ; Minami, Naojiro ; Imai, Hiroshi. / Identification and characterization of two novel classes of small RNAs in the mouse germline : Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. In: Genes and Development. 2006 ; Vol. 20, No. 13. pp. 1732-1743.
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