Identification and cloning of human G-protein γ 7, down-regulated in pancreatic cancer

Kohei Shibata, Masaki Mori, Shinji Tanaka, Seigo Kitano, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Differentially expressed genes between normal and cancer tissues of the pancreas were investigated using differential display. Consequently, we identified a fragment cDNA that was expressed in the normal tissue but was rarely expressed in the cancer tissue. This cDNA was screened in cDNA library prepared from the normal pancreatic tissue by rapid amplification of cDNA ends (5'RACE). 859 bp of cDNA was cloned and sequenced, and the inferred amino acid sequence was found to encode a G protein γ subunit with 98% homology to cow G protein γ 7 and complete homology to human G protein γ 7. The decreased expression of the G protein γ 7 was confirmed by Northern blot assay in twelve pancreatic malignancies which included nine duct cell carcinomas, two cystoadenocarcinomas and one blastoma. Reverse transcriptase (RT) -polymerase chain reaction (PCR) assay showed no expression of G protein γ 7 in five of six pancreatic carcinoma cell lines and two pancreatic cancer tissues. Immunohistochemical analysis also displayed positive staining in the normal tissue but no staining in the cancer tissue. The findings demonstrated that the reduced or suppressed expression of human G-protein γ 7 may play an important role in pancreatic carcinogenesis.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number1
DOIs
Publication statusPublished - May 8 1998

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Forensic Anthropology
Cloning
Pancreatic Neoplasms
GTP-Binding Proteins
Organism Cloning
Tissue
Complementary DNA
Assays
Cells
Staining and Labeling
Neoplasms
Polymerase chain reaction
RNA-Directed DNA Polymerase
Protein Subunits
Reverse Transcriptase Polymerase Chain Reaction
Gene Library
Northern Blotting
Ducts
Amplification
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and cloning of human G-protein γ 7, down-regulated in pancreatic cancer. / Shibata, Kohei; Mori, Masaki; Tanaka, Shinji; Kitano, Seigo; Akiyoshi, Tsuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 1, 08.05.1998, p. 205-209.

Research output: Contribution to journalArticle

Shibata, Kohei ; Mori, Masaki ; Tanaka, Shinji ; Kitano, Seigo ; Akiyoshi, Tsuyoshi. / Identification and cloning of human G-protein γ 7, down-regulated in pancreatic cancer. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 246, No. 1. pp. 205-209.
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