Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori

Kohji Yamamoto, Yoichi Aso, Hiroshi Fujii

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Abstract

The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and specifically to MCM7, of vertebrates and invertebrates. An RT-PCR showed that the bmMCM7 transcript was present in the ovaries, testes, silk glands, and fat bodies of larval silkworms. Expression plasmids were transformed into competent Escherichia coli and overexpressed. This is the first report on the identification of MCM helicase of the silkworm, B. mori.

Original languageEnglish
Article number148
JournalJournal of Insect Science
Volume10
DOIs
Publication statusPublished - Nov 29 2010

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All Science Journal Classification (ASJC) codes

  • Insect Science

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