TY - JOUR
T1 - Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori
AU - Yamamoto, Kohji
AU - Nagaoka, Sumiharu
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (KAKENHI, 15H04611) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a Research Grant for Young Investigators of the Department of Agriculture, Kyushu University.
Publisher Copyright:
© 2017, Japanese Society of Sericultural Sciences. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdyster-oids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) tech-nique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori.
AB - Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdyster-oids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) tech-nique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori.
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U2 - 10.11416/jibs.86.2_049
DO - 10.11416/jibs.86.2_049
M3 - Article
AN - SCOPUS:85026678691
VL - 86
SP - 49
EP - 53
JO - Journal of Insect Biotechnology and Sericology
JF - Journal of Insect Biotechnology and Sericology
SN - 1346-8073
IS - 2
ER -