Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori

Kohji Yamamoto; Sumiharu Nagaoka

doi:10.11416/jibs.86.2_049

Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori

Kohji Yamamoto, Sumiharu Nagaoka

Systems Biology

Research output: Contribution to journal › Article

Abstract

Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdyster-oids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) tech-nique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori.

Original language	English
Pages (from-to)	49-53
Number of pages	5
Journal	Journal of Insect Biotechnology and Sericology
Volume	86
Issue number	2
DOIs	https://doi.org/10.11416/jibs.86.2_049
Publication status	Published - Jan 1 2017

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All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

Cite this

Yamamoto, K., & Nagaoka, S. (2017). Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori. Journal of Insect Biotechnology and Sericology, 86(2), 49-53. https://doi.org/10.11416/jibs.86.2_049

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10.11416/jibs.86.2_049