Identification and molecular characterization of novel peroxidase with structural protein-like properties

Satoshi Morimoto, Norifumi Tateishi, Masako Inuyama, Futoshi Taura, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Elicitor treatment or mechanical damage to Scutellaria baicalensis Georgi (skullcap plants) callus causes an immediate insolubilization of a 36- kDa protein into cell walls. The 36-kDa protein was identified as peroxidase 1 by analysis of its internal amino acid sequence and by immunoblotting using affinity-purified anti-peroxidase 1. Insolubilized peroxidase 1 is cross- linked to lignin through covalent bonds, and the cross-linking is catalyzed in the presence of H2O2 by peroxidase 1 itself. The properties of insolubilized peroxidase 1 resemble those of defense-related structural proteins (extensins and proline-rich proteins) cross-linked to cell wall. Although the isozymes peroxidases 2 and 3 have enzyme activities similar to peroxidase 1, they are not insolubilized by stress treatment. Molecular characterization established that peroxidase 1 contains regions characteristic of structural proteins, but peroxidases 2 and 3 do not have such regions. These results suggest that among the three isozymes, only peroxidase 1 has a structural protein-like function as well as an enzymatic function.

Original languageEnglish
Pages (from-to)26192-26198
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number37
DOIs
Publication statusPublished - Sep 10 1999

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Peroxidase
Proteins
Peroxidases
Cell Wall
Isoenzymes
Scutellaria
Cells
Scutellaria baicalensis
Covalent bonds
Lignin
Bony Callus
Enzyme activity
Immunoblotting
Proline
Amino Acid Sequence
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and molecular characterization of novel peroxidase with structural protein-like properties. / Morimoto, Satoshi; Tateishi, Norifumi; Inuyama, Masako; Taura, Futoshi; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Journal of Biological Chemistry, Vol. 274, No. 37, 10.09.1999, p. 26192-26198.

Research output: Contribution to journalArticle

Morimoto, Satoshi ; Tateishi, Norifumi ; Inuyama, Masako ; Taura, Futoshi ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Identification and molecular characterization of novel peroxidase with structural protein-like properties. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 37. pp. 26192-26198.
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