Abstract
Although the liver has been known for its enormous regenerative capacity, little is known about the mechanisms responsible for such regeneration. To provide evidence for the existence of liver stem cell, using FACS and single cell-based assays, cells with multi-lineage differentiation potential and self-renewal capability have been prospectively identified. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When the cells clonally expanded in vitro were transplanted into mouse, they morphologically and functionally differentiated into hepatocytes and cholangiocytes. Furthermore, these cells differentiated into pancreatic acinar cells or intestinal epithelial cells upon transplantation into pancreas or duodenal wall. Manipulation of self-renewing liver stem cells may provide new insight into therapies for diseases of the digestive system.
| Original language | English |
|---|---|
| Pages (from-to) | 455-461 |
| Number of pages | 7 |
| Journal | Seminars in Cell and Developmental Biology |
| Volume | 13 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jan 1 2002 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Developmental Biology
- Cell Biology
Cite this
Identification and propagation of liver stem cells. / Suzuki, Atsushi; Nakauchi, Hiromitsu.
In: Seminars in Cell and Developmental Biology, Vol. 13, No. 6, 01.01.2002, p. 455-461.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Identification and propagation of liver stem cells
AU - Suzuki, Atsushi
AU - Nakauchi, Hiromitsu
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Although the liver has been known for its enormous regenerative capacity, little is known about the mechanisms responsible for such regeneration. To provide evidence for the existence of liver stem cell, using FACS and single cell-based assays, cells with multi-lineage differentiation potential and self-renewal capability have been prospectively identified. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When the cells clonally expanded in vitro were transplanted into mouse, they morphologically and functionally differentiated into hepatocytes and cholangiocytes. Furthermore, these cells differentiated into pancreatic acinar cells or intestinal epithelial cells upon transplantation into pancreas or duodenal wall. Manipulation of self-renewing liver stem cells may provide new insight into therapies for diseases of the digestive system.
AB - Although the liver has been known for its enormous regenerative capacity, little is known about the mechanisms responsible for such regeneration. To provide evidence for the existence of liver stem cell, using FACS and single cell-based assays, cells with multi-lineage differentiation potential and self-renewal capability have been prospectively identified. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When the cells clonally expanded in vitro were transplanted into mouse, they morphologically and functionally differentiated into hepatocytes and cholangiocytes. Furthermore, these cells differentiated into pancreatic acinar cells or intestinal epithelial cells upon transplantation into pancreas or duodenal wall. Manipulation of self-renewing liver stem cells may provide new insight into therapies for diseases of the digestive system.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036929052&partnerID=8YFLogxK
U2 - 10.1016/S1084952102001349
DO - 10.1016/S1084952102001349
M3 - Review article
C2 - 12468247
AN - SCOPUS:0036929052
VL - 13
SP - 455
EP - 461
JO - Seminars in Cell and Developmental Biology
JF - Seminars in Cell and Developmental Biology
SN - 1084-9521
IS - 6
ER -