Identification, characterization and interaction of HAP family genes in rice

Thiruvengadam Thirumurugan, Yukihiro Ito, Takahiko Kubo, Akiko Serizawa, Nori Kurata

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

Original languageEnglish
Pages (from-to)279-289
Number of pages11
JournalMolecular Genetics and Genomics
Volume279
Issue number3
DOIs
Publication statusPublished - Mar 1 2008
Externally publishedYes

Fingerprint

Genes
Oryza
Protein Subunits
Growth and Development
Amino Acid Sequence
Proteins
Genome
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Identification, characterization and interaction of HAP family genes in rice. / Thirumurugan, Thiruvengadam; Ito, Yukihiro; Kubo, Takahiko; Serizawa, Akiko; Kurata, Nori.

In: Molecular Genetics and Genomics, Vol. 279, No. 3, 01.03.2008, p. 279-289.

Research output: Contribution to journalArticle

Thirumurugan, Thiruvengadam ; Ito, Yukihiro ; Kubo, Takahiko ; Serizawa, Akiko ; Kurata, Nori. / Identification, characterization and interaction of HAP family genes in rice. In: Molecular Genetics and Genomics. 2008 ; Vol. 279, No. 3. pp. 279-289.
@article{49ea06d99dac41248a53c4e95dfd830f,
title = "Identification, characterization and interaction of HAP family genes in rice",
abstract = "A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.",
author = "Thiruvengadam Thirumurugan and Yukihiro Ito and Takahiko Kubo and Akiko Serizawa and Nori Kurata",
year = "2008",
month = "3",
day = "1",
doi = "10.1007/s00438-007-0312-3",
language = "English",
volume = "279",
pages = "279--289",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Identification, characterization and interaction of HAP family genes in rice

AU - Thirumurugan, Thiruvengadam

AU - Ito, Yukihiro

AU - Kubo, Takahiko

AU - Serizawa, Akiko

AU - Kurata, Nori

PY - 2008/3/1

Y1 - 2008/3/1

N2 - A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

AB - A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

UR - http://www.scopus.com/inward/record.url?scp=39249083844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39249083844&partnerID=8YFLogxK

U2 - 10.1007/s00438-007-0312-3

DO - 10.1007/s00438-007-0312-3

M3 - Article

VL - 279

SP - 279

EP - 289

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 3

ER -