Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium

Kohei Himeno, K. Johan Rosengren, Tomoko Inoue, Rodney H. Perez, Michelle L. Colgrave, Han Siean Lee, Lai Y. Chan, Sónia Troeira Henriques, Koji Fujita, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Jiro Nakayama, Vichien Leelawatcharamas, Hiroyuki Jikuya, David J. Craik, Kenji Sonomoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu24 and Trp87 to yield the mature (circular) enterocin NKR-5-3B.

Original languageEnglish
Pages (from-to)4863-4876
Number of pages14
JournalBiochemistry
Volume54
Issue number31
DOIs
Publication statusPublished - Aug 11 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry

Fingerprint Dive into the research topics of 'Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium'. Together they form a unique fingerprint.

  • Cite this

    Himeno, K., Rosengren, K. J., Inoue, T., Perez, R. H., Colgrave, M. L., Lee, H. S., Chan, L. Y., Henriques, S. T., Fujita, K., Ishibashi, N., Zendo, T., Wilaipun, P., Nakayama, J., Leelawatcharamas, V., Jikuya, H., Craik, D. J., & Sonomoto, K. (2015). Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium. Biochemistry, 54(31), 4863-4876. https://doi.org/10.1021/acs.biochem.5b00196