Identification of a cDNA encoding a thiazide-sensitive sodium-chloride cotransporter from the human and its mRNA expression in various tissues

Hangil Chang, Kosuke Tashiro, Momoki Hirai, Kyoji Ikeda, Kiyoshi Kurokawa, Toshiro Fujita

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We report here the identification of a cDNA encoding a human thiazide-sensitive sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at the nucleotide level, and 92% and 61%, respectively, at the amino acid level. Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to chromosome 16q13. Northern blot analysis using polyA+RNA from various human tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in the kidney, but low level of expression was also observed in small intestine, placenta, prostate, colon, and spleen.

Original languageEnglish
Pages (from-to)324-328
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume223
Issue number2
DOIs
Publication statusPublished - Jun 14 1996
Externally publishedYes

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Member 3 Solute Carrier Family 12
Complementary DNA
Tissue
Messenger RNA
Chromosomes
Rats
Nucleotides
Genes
Fluorescence
Fluorescence In Situ Hybridization
Northern Blotting
RNA
Placenta
Small Intestine
Amino Acids
Prostate
Colon
Spleen
Kidney
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of a cDNA encoding a thiazide-sensitive sodium-chloride cotransporter from the human and its mRNA expression in various tissues. / Chang, Hangil; Tashiro, Kosuke; Hirai, Momoki; Ikeda, Kyoji; Kurokawa, Kiyoshi; Fujita, Toshiro.

In: Biochemical and Biophysical Research Communications, Vol. 223, No. 2, 14.06.1996, p. 324-328.

Research output: Contribution to journalArticle

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