Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER

Abbas Shakoori, Gourou Fujii, Shin Ichiro Yoshimura, Masashi Kitamura, Kazuhisa Nakayama, Takashi Ito, Hiroshi Ohno, Nobuhiro Nakamura

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A family of five-pass transmembrane proteins (FinGERs) were identified from the protein sequence database. The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species. Eight family members were found in human. Multiple sequence alignment revealed three regions conserved among all family members. All of the human family members were expressed widely in various tissues. The human proteins were localized in and around the Golgi apparatus and may also be in the ER to some extent. The Golgi apparatus was fragmented by overexpression of the five of the family members. Some of the members were found to interact by yeast two-hybrid analysis, suggesting the formation of a complex. These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus.

Original languageEnglish
Pages (from-to)850-857
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume312
Issue number3
DOIs
Publication statusPublished - Dec 19 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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