Abstract
We had isolated three genes (rmlA, rmlB, and rmlC) involved in dTDP- rhamnose synthesis in Streptococcus mutans and found that three genes were insufficient for dTDP-rhamnose synthesis (Y. Tsukioka, Y. Yamashita, T. Oho, Y. Nakano, and T. Koga, J. Bacteriol. 179:1126-1134, 1997). The rmlD gene of S. mutans, encoding the enzyme which catalyzes the last step of dTDP- rhamnose synthesis, has been cloned and sequenced. The cell extract of Escherichia coli expressing the rmlD gene of S. mutans exhibited enzymatic activity corresponding to its counterpart in Shigella flexneri, a gram- negative bacterium. Rhamnose was not detected in the cell wall preparation purified from the mutant in which the cloned gene was insertionally inactivated. Rabbit antiserum against S. mutans serotype c-specific antigen did not react with autoclaved extracts from the mutant. The rmlD gene product of S. mutans compensated for the incompleteness of dTDP-rhamnose synthesis by the three previously isolated genes. These results indicate that the rmlD gene product is indispensable for the dTDP-rhamnose pathway and subsequently far the synthesis of serotype-specific antigen in S. mutans. Furthermore, conservation of the rmlD gene in Streptococcus species was demonstrated by Southern blot analysis.
Original language | English |
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Pages (from-to) | 4411-4414 |
Number of pages | 4 |
Journal | Journal of bacteriology |
Volume | 179 |
Issue number | 13 |
DOIs | |
Publication status | Published - Jan 1 1997 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
Cite this
Identification of a fourth gene involved in dTDP-rhamnose synthesis in Streptococcus mutans. / Tsukioka, Y.; Yamashita, Y.; Nakano, Y.; Oho, T.; Koga, T.
In: Journal of bacteriology, Vol. 179, No. 13, 01.01.1997, p. 4411-4414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a fourth gene involved in dTDP-rhamnose synthesis in Streptococcus mutans
AU - Tsukioka, Y.
AU - Yamashita, Y.
AU - Nakano, Y.
AU - Oho, T.
AU - Koga, T.
PY - 1997/1/1
Y1 - 1997/1/1
N2 - We had isolated three genes (rmlA, rmlB, and rmlC) involved in dTDP- rhamnose synthesis in Streptococcus mutans and found that three genes were insufficient for dTDP-rhamnose synthesis (Y. Tsukioka, Y. Yamashita, T. Oho, Y. Nakano, and T. Koga, J. Bacteriol. 179:1126-1134, 1997). The rmlD gene of S. mutans, encoding the enzyme which catalyzes the last step of dTDP- rhamnose synthesis, has been cloned and sequenced. The cell extract of Escherichia coli expressing the rmlD gene of S. mutans exhibited enzymatic activity corresponding to its counterpart in Shigella flexneri, a gram- negative bacterium. Rhamnose was not detected in the cell wall preparation purified from the mutant in which the cloned gene was insertionally inactivated. Rabbit antiserum against S. mutans serotype c-specific antigen did not react with autoclaved extracts from the mutant. The rmlD gene product of S. mutans compensated for the incompleteness of dTDP-rhamnose synthesis by the three previously isolated genes. These results indicate that the rmlD gene product is indispensable for the dTDP-rhamnose pathway and subsequently far the synthesis of serotype-specific antigen in S. mutans. Furthermore, conservation of the rmlD gene in Streptococcus species was demonstrated by Southern blot analysis.
AB - We had isolated three genes (rmlA, rmlB, and rmlC) involved in dTDP- rhamnose synthesis in Streptococcus mutans and found that three genes were insufficient for dTDP-rhamnose synthesis (Y. Tsukioka, Y. Yamashita, T. Oho, Y. Nakano, and T. Koga, J. Bacteriol. 179:1126-1134, 1997). The rmlD gene of S. mutans, encoding the enzyme which catalyzes the last step of dTDP- rhamnose synthesis, has been cloned and sequenced. The cell extract of Escherichia coli expressing the rmlD gene of S. mutans exhibited enzymatic activity corresponding to its counterpart in Shigella flexneri, a gram- negative bacterium. Rhamnose was not detected in the cell wall preparation purified from the mutant in which the cloned gene was insertionally inactivated. Rabbit antiserum against S. mutans serotype c-specific antigen did not react with autoclaved extracts from the mutant. The rmlD gene product of S. mutans compensated for the incompleteness of dTDP-rhamnose synthesis by the three previously isolated genes. These results indicate that the rmlD gene product is indispensable for the dTDP-rhamnose pathway and subsequently far the synthesis of serotype-specific antigen in S. mutans. Furthermore, conservation of the rmlD gene in Streptococcus species was demonstrated by Southern blot analysis.
UR - http://www.scopus.com/inward/record.url?scp=0030747366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030747366&partnerID=8YFLogxK
U2 - 10.1128/jb.179.13.4411-4414.1997
DO - 10.1128/jb.179.13.4411-4414.1997
M3 - Article
C2 - 9209063
AN - SCOPUS:0030747366
VL - 179
SP - 4411
EP - 4414
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 13
ER -