Identification of a Methylated Tea Catechin as an Inhibitor of Degranulation in Human Basophilic KU812 Cells

Hirofumi Tachibana, Yousuke Sunada, Toshio Miyase, Mitsuaki Sano, Mari Maeda-Yamamoto, Koji Yamada

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We examined the constituents of tea that had an inhibitory effect on the degranulation process in the human basophilic cell line, KU812. Among the constituents purified from a extract of ‘Benihomare’ oolong tea by column chromatography, a methylated (-)-epigallocatechin gallate ((-)-epigallocatechin 3-O-(3-O-methyl) gallate) was found to inhibit the degranulation of KU812 cells that had been stimulated with calcium ionophore A23187. The inhibitory effect of this methylated (-)-epigallocatechin gallate on degranulation was greater than that of (-)-epigallocatechin gallate. This result indicates that methylation of (-)-epigallocatechin gallate may be an effective modification for the catechin to inhibit degranulation from human basophils.

Original languageEnglish
Pages (from-to)452-454
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number2
DOIs
Publication statusPublished - Jan 1 2000

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Catechin
Tea
Cell Degranulation
Column chromatography
Methylation
Basophils
Calcium Ionophores
Calcimycin
Chromatography
Cells
Cell Line
epigallocatechin gallate

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Identification of a Methylated Tea Catechin as an Inhibitor of Degranulation in Human Basophilic KU812 Cells. / Tachibana, Hirofumi; Sunada, Yousuke; Miyase, Toshio; Sano, Mitsuaki; Maeda-Yamamoto, Mari; Yamada, Koji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 2, 01.01.2000, p. 452-454.

Research output: Contribution to journalArticle

Tachibana, Hirofumi ; Sunada, Yousuke ; Miyase, Toshio ; Sano, Mitsuaki ; Maeda-Yamamoto, Mari ; Yamada, Koji. / Identification of a Methylated Tea Catechin as an Inhibitor of Degranulation in Human Basophilic KU812 Cells. In: Bioscience, Biotechnology and Biochemistry. 2000 ; Vol. 64, No. 2. pp. 452-454.
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