Abstract
We examined the constituents of tea that had an inhibitory effect on the degranulation process in the human basophilic cell line, KU812. Among the constituents purified from a extract of ‘Benihomare’ oolong tea by column chromatography, a methylated (-)-epigallocatechin gallate ((-)-epigallocatechin 3-O-(3-O-methyl) gallate) was found to inhibit the degranulation of KU812 cells that had been stimulated with calcium ionophore A23187. The inhibitory effect of this methylated (-)-epigallocatechin gallate on degranulation was greater than that of (-)-epigallocatechin gallate. This result indicates that methylation of (-)-epigallocatechin gallate may be an effective modification for the catechin to inhibit degranulation from human basophils.
| Original language | English |
|---|---|
| Pages (from-to) | 452-454 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 64 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Identification of a Methylated Tea Catechin as an Inhibitor of Degranulation in Human Basophilic KU812 Cells. / Tachibana, Hirofumi; Sunada, Yousuke; Miyase, Toshio; Sano, Mitsuaki; Maeda-Yamamoto, Mari; Yamada, Koji.
In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 2, 01.01.2000, p. 452-454.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of a Methylated Tea Catechin as an Inhibitor of Degranulation in Human Basophilic KU812 Cells
AU - Tachibana, Hirofumi
AU - Sunada, Yousuke
AU - Miyase, Toshio
AU - Sano, Mitsuaki
AU - Maeda-Yamamoto, Mari
AU - Yamada, Koji
PY - 2000/1/1
Y1 - 2000/1/1
N2 - We examined the constituents of tea that had an inhibitory effect on the degranulation process in the human basophilic cell line, KU812. Among the constituents purified from a extract of ‘Benihomare’ oolong tea by column chromatography, a methylated (-)-epigallocatechin gallate ((-)-epigallocatechin 3-O-(3-O-methyl) gallate) was found to inhibit the degranulation of KU812 cells that had been stimulated with calcium ionophore A23187. The inhibitory effect of this methylated (-)-epigallocatechin gallate on degranulation was greater than that of (-)-epigallocatechin gallate. This result indicates that methylation of (-)-epigallocatechin gallate may be an effective modification for the catechin to inhibit degranulation from human basophils.
AB - We examined the constituents of tea that had an inhibitory effect on the degranulation process in the human basophilic cell line, KU812. Among the constituents purified from a extract of ‘Benihomare’ oolong tea by column chromatography, a methylated (-)-epigallocatechin gallate ((-)-epigallocatechin 3-O-(3-O-methyl) gallate) was found to inhibit the degranulation of KU812 cells that had been stimulated with calcium ionophore A23187. The inhibitory effect of this methylated (-)-epigallocatechin gallate on degranulation was greater than that of (-)-epigallocatechin gallate. This result indicates that methylation of (-)-epigallocatechin gallate may be an effective modification for the catechin to inhibit degranulation from human basophils.
UR - http://www.scopus.com/inward/record.url?scp=0034133254&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034133254&partnerID=8YFLogxK
U2 - 10.1271/bbb.64.452
DO - 10.1271/bbb.64.452
M3 - Article
C2 - 10737211
AN - SCOPUS:0034133254
VL - 64
SP - 452
EP - 454
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -