Identification of a new sprouty protein responsible for the inhibition of the Bombyx mori nucleopolyhedrovirus reproduction

Shengkai Jin, Tingcai Cheng, Liang Jiang, Ping Lin, Qiong Yang, Yang Xiao, Takahiro Kusakabe, Qingyou Xia

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The rat sarcoma-extracellular signal regulated kinase mitogen-activated protein kinases pathway, one of the most ancient signaling pathways, is crucial for the defense against Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Sprouty (Spry) proteins can inhibit the activity of this pathway by receptor tyrosine kinases. We cloned and identified a new B. mori gene with a Spry domain similar to the Spry proteins of other organisms, such as fruitfly, mouse, human, chicken, Xenopus and zebrafish, and named it BmSpry. The gene expression analysis showed that BmSpry was transcribed in all of the examined tissues and in all developmental stages from embryo to adult. BmSpry also induced expression of BmNPV in the cells. Our results indicated: (1) the knock-down of BmSpry led to increased BmNPV replication and silkworm larvae mortality; (2) overexpression of BmSpry led to reduced BmNPV replication; and (3) BmSpry regulated the activation of ERK and inhibited BmNPV replication. These results showed that BmSpry plays a crucial role in the antiviral defense of the silkworm both in vitro and in vivo.

Original languageEnglish
Article numbere99200
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 10 2014

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Bombyx mori nucleopolyhedrovirus
Nucleopolyhedrovirus
Bombyx
Reproduction
Extracellular Signal-Regulated MAP Kinases
Receptor Protein-Tyrosine Kinases
Mitogen-Activated Protein Kinases
Gene expression
Antiviral Agents
Rats
Proteins
mitogen-activated protein kinase
proteins
Genes
Chemical activation
Tissue
mitogen-activated protein kinase kinase
sarcoma
Xenopus
Bombyx mori

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Identification of a new sprouty protein responsible for the inhibition of the Bombyx mori nucleopolyhedrovirus reproduction. / Jin, Shengkai; Cheng, Tingcai; Jiang, Liang; Lin, Ping; Yang, Qiong; Xiao, Yang; Kusakabe, Takahiro; Xia, Qingyou.

In: PloS one, Vol. 9, No. 6, e99200, 10.06.2014.

Research output: Contribution to journalArticle

Jin, Shengkai ; Cheng, Tingcai ; Jiang, Liang ; Lin, Ping ; Yang, Qiong ; Xiao, Yang ; Kusakabe, Takahiro ; Xia, Qingyou. / Identification of a new sprouty protein responsible for the inhibition of the Bombyx mori nucleopolyhedrovirus reproduction. In: PloS one. 2014 ; Vol. 9, No. 6.
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