Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29

Nozomi Ishijima, Ken Ichi Lee, Tomomi Kuwahara, Haruyuki Nakayama-Imaohji, Saori Yoneda, Atsushi Iguchi, Yoshitoshi Ogura, Tetsuya Hayashi, Makoto Ohnishi, Sunao Iyoda

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Abstract

Enterohemorrhagic Escherichia coli (EHEC) O26 infections cause severe human diseases such as hemolytic uremic syndrome and encephalopathy, and is the predominant serogroup among non-O157 EHEC in many countries. Shiga toxin (Stx), which consists of two distinct types (Stx1 and Stx2), plays a central role in EHEC pathogenesis. The major stx gene type in EHEC O26 strains is stx1, although isolates with only stx2 have emerged in Japan since 2012 and have been reported in Europe. In this study, we selected 27 EHEC O26 strains isolated in Japan and identified a distinct genetic clade within sequence type (ST) 29, designated ST29C1, that carried only stx2 and had the plasmid gene profile ehxA. We showed that ST29C1 strains produced higher Stx2a levels, and greater virulence in Vero cells and in germ-free mice than other lineages. We also showed that ST29C1 was a distinct phylogenetic clade by SNP analysis using whole genome sequences and clearly differed from the major European EHEC O26 virulent clone, which was designated ST29C2 in this study. The combination of toxin production analysis, virulence analysis in Vero cells and germ-free mice, and phylogenetic analysis identified a newly emerging virulent EHEC clade.

Original languageEnglish
Article number43136
JournalScientific reports
Volume7
DOIs
Publication statusPublished - Feb 23 2017

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Enterohemorrhagic Escherichia coli
Vero Cells
Virulence
Japan
Shiga Toxin
Escherichia coli Infections
Hemolytic-Uremic Syndrome
Brain Diseases
Genes
Single Nucleotide Polymorphism
Plasmids
Clone Cells
Genome

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ishijima, N., Lee, K. I., Kuwahara, T., Nakayama-Imaohji, H., Yoneda, S., Iguchi, A., ... Iyoda, S. (2017). Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29. Scientific reports, 7, [43136]. https://doi.org/10.1038/srep43136

Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29. / Ishijima, Nozomi; Lee, Ken Ichi; Kuwahara, Tomomi; Nakayama-Imaohji, Haruyuki; Yoneda, Saori; Iguchi, Atsushi; Ogura, Yoshitoshi; Hayashi, Tetsuya; Ohnishi, Makoto; Iyoda, Sunao.

In: Scientific reports, Vol. 7, 43136, 23.02.2017.

Research output: Contribution to journalArticle

Ishijima, N, Lee, KI, Kuwahara, T, Nakayama-Imaohji, H, Yoneda, S, Iguchi, A, Ogura, Y, Hayashi, T, Ohnishi, M & Iyoda, S 2017, 'Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29', Scientific reports, vol. 7, 43136. https://doi.org/10.1038/srep43136
Ishijima N, Lee KI, Kuwahara T, Nakayama-Imaohji H, Yoneda S, Iguchi A et al. Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29. Scientific reports. 2017 Feb 23;7. 43136. https://doi.org/10.1038/srep43136
Ishijima, Nozomi ; Lee, Ken Ichi ; Kuwahara, Tomomi ; Nakayama-Imaohji, Haruyuki ; Yoneda, Saori ; Iguchi, Atsushi ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Ohnishi, Makoto ; Iyoda, Sunao. / Identification of a new virulent clade in enterohemorrhagic escherichia coli O26:H11/H- Sequence Type 29. In: Scientific reports. 2017 ; Vol. 7.
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