Identification of a novel human cancer/testis antigen, KM-HN-1, recognized by cellular and humoral immune responses

Mikio Monji, Tetsuya Nakatsura, Satoru Senju, Yoshihiro Yoshitake, Motohiro Sawatsubashi, Masanori Shinohara, Toshiro Kageshita, Tomomichi Ono, Akira Inokuchi, Yasuharu Nishimura

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Purpose: We used serologic screening of a cDNA expression library of human testis to identify novel cancer/testis antigens that elicit both humoral and cellular immune responses in cancer patients. Experimental Design and Results: We identified a novel gene designated KM-HN-1 the expression of which is testis-specific among normal tissues; it contains coiled coil domains and a leucine zipper motif and encodes a putative protein consisting of 833 amino acids. KM-HN-1 expression was observed in various cancer tissues and cancer cell lines at both mRNA and protein levels. Immunofluorescence staining of an esophageal cancer cell line revealed that KM-HN-1 protein was present exclusively in the nucleus during mitosis. Recombinant KM-HN-1 protein was produced, and used for ELISA to quantitate levels of IgG antibody specific to KM-HN-1. Higher levels of IgG antibodies specific to KM-HN-1 were detected in many types and numbers of cancer patients but not in healthy donors. The CTL lines specific to KM-HN-1, generated from HLA-A*2402-positive healthy donors and cancer patients, killed human leukocyte antigen (HLA)-A24-positive cancer cells expressing KM-HN-1 but not cell lines that did not express either KM-HN-1 or HLA-A24. Conclusions: We identified a novel cancer/testis antigen, KM-HN-1, which elicited humoral immune responses in patients with various types of cancer. Furthermore, KM-HN-1-specific CTLs could be generated from both healthy donors and cancer patients, which indicated that KM-HN-1 can be a candidate for an ideal target for cancer immunotherapy.

Original languageEnglish
Pages (from-to)6047-6057
Number of pages11
JournalClinical Cancer Research
Volume10
Issue number18 I
DOIs
Publication statusPublished - Sep 15 2004

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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