Identification of a novel isoform of ZAP-70, truncated ZAP kinase

Hiroyuki Kuroyama, Tohru Ikeda, Michiyuki Kasai, Shou Yamasaki, Masashi Tatsumi, Masanori Utsuyama, Takashi Saito, Katsuiku Hirokawa

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    We identified a novel cDNA encoding truncated ZAP-70, which lacked the SH2 domain and a part of interdomain B, and named it truncated ZAP kinase (TZK). TZK was expressed in the thymus, spleen, and lymph nodes with ZAP-70. TZK was expressed in CD44+CD25- thymocytes up to mature T cells, but ZAP-70 was not expressed in CD44+CD25- or CD44 +CD25+ thymocytes. ZAP-70 or TZK was transfected into P116 cells derived from a Jurkat T-cell line deficient in ZAP-70. The P116 cells with ZAP-70 induced the T-cell receptor-mediated signal transduction, but the cells expressing TZK did not. While ZAP-70 was accumulated at the immune synapse, TZK was not. Meanwhile, impaired phosphorylation of SLP-76, one of the substrates of ZAP-70, in P116 cells upon pervanadate stimulation was rescued in the cells expressing TZK. These findings show that TZK is a novel isoform of ZAP-70, which is expressed in pre-T-cell receptor-minus thymocytes and functions as a kinase not associated with T-cell receptor.

    Original languageEnglish
    Pages (from-to)935-941
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume315
    Issue number4
    DOIs
    Publication statusPublished - Mar 19 2004

    Fingerprint

    ZAP-70 Protein-Tyrosine Kinase
    Protein Isoforms
    Phosphotransferases
    Thymocytes
    T-Cell Antigen Receptor
    T-cells
    Cells
    T-Lymphocytes
    Thymus
    Signal transduction
    Phosphorylation
    Jurkat Cells
    src Homology Domains
    Synapses
    Thymus Gland
    Signal Transduction
    Spleen
    Complementary DNA
    Lymph Nodes

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Kuroyama, H., Ikeda, T., Kasai, M., Yamasaki, S., Tatsumi, M., Utsuyama, M., ... Hirokawa, K. (2004). Identification of a novel isoform of ZAP-70, truncated ZAP kinase. Biochemical and Biophysical Research Communications, 315(4), 935-941. https://doi.org/10.1016/j.bbrc.2004.01.127

    Identification of a novel isoform of ZAP-70, truncated ZAP kinase. / Kuroyama, Hiroyuki; Ikeda, Tohru; Kasai, Michiyuki; Yamasaki, Shou; Tatsumi, Masashi; Utsuyama, Masanori; Saito, Takashi; Hirokawa, Katsuiku.

    In: Biochemical and Biophysical Research Communications, Vol. 315, No. 4, 19.03.2004, p. 935-941.

    Research output: Contribution to journalArticle

    Kuroyama, H, Ikeda, T, Kasai, M, Yamasaki, S, Tatsumi, M, Utsuyama, M, Saito, T & Hirokawa, K 2004, 'Identification of a novel isoform of ZAP-70, truncated ZAP kinase', Biochemical and Biophysical Research Communications, vol. 315, no. 4, pp. 935-941. https://doi.org/10.1016/j.bbrc.2004.01.127
    Kuroyama, Hiroyuki ; Ikeda, Tohru ; Kasai, Michiyuki ; Yamasaki, Shou ; Tatsumi, Masashi ; Utsuyama, Masanori ; Saito, Takashi ; Hirokawa, Katsuiku. / Identification of a novel isoform of ZAP-70, truncated ZAP kinase. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 315, No. 4. pp. 935-941.
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