Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor

Taiju Amano, Shunsuke Aoki, Rieko Setsuie, Mikako Sakurai, Keiji Wada, Mami Noda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The norepinephrine transporter (NET) plays a crucial role in noradrenergic neurotransmission and is a target of many antidepressants and psychostimulants. Intracellular Ca2+ is reportedly involved in regulating NET activity, but the detailed mechanism is not clear. We employed a norepinephrine uptake assay using SH-SY5Y cells and found that the IP3 receptor inhibitors, 2-aminoethoxydiphenyl borate and xestospongin C, reduced the NET Vmax. These reductions were accompanied by the decreased cell surface expression of NET. Our findings suggest that intracellular Ca2+ mobilized by IP3 receptor is required for the maintenance of NET activity. This adds another pathway involving Ca2+ for the regulation of NET to other known mechanisms providing intracellular Ca2+.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalEuropean Journal of Pharmacology
Volume536
Issue number1-2
DOIs
Publication statusPublished - Apr 24 2006

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Norepinephrine Plasma Membrane Transport Proteins
Inositol 1,4,5-Trisphosphate Receptors
Synaptic Transmission
Antidepressive Agents
Norepinephrine
Maintenance

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor. / Amano, Taiju; Aoki, Shunsuke; Setsuie, Rieko; Sakurai, Mikako; Wada, Keiji; Noda, Mami.

In: European Journal of Pharmacology, Vol. 536, No. 1-2, 24.04.2006, p. 62-68.

Research output: Contribution to journalArticle

Amano, Taiju ; Aoki, Shunsuke ; Setsuie, Rieko ; Sakurai, Mikako ; Wada, Keiji ; Noda, Mami. / Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor. In: European Journal of Pharmacology. 2006 ; Vol. 536, No. 1-2. pp. 62-68.
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